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不同的机制使Schoenoplectiella juncoides (Roxb.) 具有对硫甲基的耐药性. 子子子子子

Liru Liu¹, Yize Zou¹, Yidi Guan¹

¹College of Plant Protection, Shenyang Agricultural University, No. 120 Dongling Road, Shenhe District, Shenyang City 110866, China.

Pesticide biochemistry and physiology

|September 14, 2024

概括

在中国,大米杂草中的硫甲基耐药性Schoenoplectiella juncoides正在增加. 这项研究发现,点突变和增强的新陈代谢都对这种除草剂耐药性有贡献.

科学领域:

农业科学农业科学
植物科学植物科学
杂草科学杂草科学

背景情况:

在中国大米田中,硫甲基对Schoenoplectiella juncoides的疗效降低.
需要了解这一关键杂草物种进化的除草剂耐药性背后的机制.

研究的目的:

研究一种抗硫甲基耐药的Schoenoplectiella juncoides种群 (W15) 的耐药机制.
将W15与易受感染的人群 (W4) 进行比较,以阐明耐药性演变.

主要方法:

全植物剂量反应实验和ALS活性测定.
对ALS基因和基因表达的分子分析.
液体染色学-并联质谱学 (LC-MS/MS) 用于代谢分析.
酶活性测定 (谷氨S转移酶).

主要成果:

W15对硫甲基表现出高水平的耐药性,由剂量反应和ALS活性证实.
在W15中确定了ALS1中的Pro-197-Ser突变;在ALS基因表达中没有显著差异.
W15表现出硫甲基代谢的增加,耐药性因马拉和NBD-Cl.Cl而减轻.
在W15中观察到高Glutathione S-转移酶活性和对其他除草剂的交叉耐药性.

结论:

目标部位 (ALS1中的Pro-197-Ser突变) 和非目标部位 (通过GSTs增强新陈代谢) 的机制都对S. juncoides的硫甲基耐药性有所贡献.
这种双重机制的抗性对大米生产中的杂草管理构成了重大挑战.

关键词:

乙酸乳酸合成酶突变的发生硫甲基 - 硫甲基的使用代谢阻力代谢阻力代谢阻力对多种除草剂产生耐药性.状 (Roxb.) 状 (Schoenoplectiella juncoides) (Roxb.) 状 (Roxb.) 是一种状的植物. 子子子子子

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