Lnc557通过与BmELAVL1相互作用来促进Bombyx mori核多重体病毒的复制,以提高其稳定性和表达
Su Lin1, Zhen-Yu Shen1, Meng-Dong Wang1
1Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.
Pesticide biochemistry and physiology
|September 14, 2024
概括
一种新发现的长非编码RNA, lnc557,通过稳定BmELAVL1蛋白质来促进Bombyx mori核多重体病毒 (BmNPV) 复制,为昆虫病毒相互作用提供了新的见解.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 昆虫科学 昆虫科学 昆虫科学
背景情况:
- 博姆比克斯·莫里核多重体病毒 (BmNPV) 显著影响到丝养殖产业.
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在病毒与宿主相互作用中的角色,但它们在昆虫病毒系统中的特定功能仍未得到充分探索.
研究的目的:
- 研究lncRNAs在Bombyx mori和BmNPV之间的相互作用中的作用.
- 确定特定的lncRNA及其参与调节病毒复制的蛋白标.
主要方法:
- 转录组测序和核糖体分析测序 (Ribo-seq) 用于BmNPV感染和控制丝虫中肠组织.
- 生物信息分析确定了差异表达的 lncRNA 和小型开放读取框架 (sORF).
- 实验验证包括亚细胞局部化,基因过度表达/敲击,RNA下拉,质谱,蛋白质截断和RNA免疫沉 (RIP) 试验.
主要成果:
- 确定了9个差异表达的lncRNA和27个sORF. 在BmNPV感染后,LncRNA XR_001139971.3 (lnc557) 显著上调.
- 发现局部在细胞质中的Lnc557在过度表达时促进BmNPV复制.
- Lnc557直接与BmELAVL1蛋白的RRM-5域结合,增强其稳定性和表达.
- 增加BmELAVL1的表达促进了BmNPV的扩散,而它的抑制则具有抑制作用.
结论:
- Lnc557在通过BmELAVL1途径BmNPV复制中发挥着积极的调节作用.
- 这项研究揭示了Bombyx mori-BmNPV相互作用中的一种新型分子机制.
- 这些发现为控制养殖中的BmNPV提供了新的目标.
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