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在Eleusine indica中对葡萄酸盐的代谢抵抗机制

Tianhong Lei¹, Tangqi Feng¹, Lei Wang¹

¹National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Plant Science & Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Pesticide biochemistry and physiology

|September 14, 2024

概括

在Eleusine indica中对葡萄酸盐的代谢耐药性与增加的谷氨S转移酶 (GST) 活性有关. 特定的基因GSTU6和GST4被上调,这表明它们在这种麻烦的杂草中对除草剂耐药性的作用.

科学领域:

农业科学农业科学
植物科学植物科学
生物化学生物化学

背景情况:

印度草 (Eleusine indica) 是全球农业的主要杂草,特别是在中国的果园里.
葡萄酸盐是用于控制E. indica的广泛使用的除草剂,但耐药性是一个越来越令人担忧的问题.

研究的目的:

调查来自中国果园的E. indica种群中葡萄酸盐耐药性的机制.
为了确定谷氨酸合成酶 (GS) 的目标位点突变或涉及谷氨酸S转移酶 (GST) 的代谢变化是否负责耐药性.

主要方法:

进行了生物测试,以量化葡萄酸盐耐药性水平.
分析了谷氨酸合成酶 (GS) 的活性和基因表达.
测量了谷氨S转移酶 (GST) 活性,并评估了GST抑制剂的作用.
用RNA测序 (RNA-seq) 来分析基因表达特征.

主要成果:

耐药 (R) E. indica 种群与敏感 (S) 种群相比,对葡萄酸盐产生了3倍的耐药性.
在R和S植物之间没有发现GS活性或已知的耐药性相关突变的显著差异.
在R植物中,GST活性显著更高,GST抑制剂增强了对R植物的葡萄酸盐有效性.
在R植物中,RNA-seq显示了GSTU6和GST4基因表达的显著上调,无论是用葡萄酸盐治疗还是没有治疗.

结论:

在这种E. indica种群中,葡萄糖酸盐耐药性并不是由于GS的目标部位不敏感.
涉及增强的谷氨S转移酶 (GST) 活动的代谢抵抗是主要的机制.
GSTU6和GST4基因可能是E. indica. 葡萄糖酸代谢耐药性的关键贡献者.

关键词:

埃卢辛 (Eleusine) 指标是一种指标.葡萄糖酸盐的使用方法谷氨酸S转移酶是一种S转移酶.对新陈代谢产生抵抗力.在RNA-seqqq.

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