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在Bombyx mori中,cyantraniliprole诱导的丝腺和转录组异常的发展

Xiaohan Liu¹, Ruinan Qi¹, Fanchi Li²

¹School of Life Sciences, Suzhou Medical College of Soochow University, Suzhou, Jiangsu 215123, PR China.

Pesticide biochemistry and physiology

|September 14, 2024

概括

昆虫杀虫剂 (Cya) 伤害Bombyx mori丝腺,延迟发育并降低子质量. 干扰细胞过程,如DNA复制和蛋白质合成在丝腺.

科学领域:

昆虫学昆虫学是一门学科.
毒理学毒理学毒理学
生物化学生物化学

背景情况:

波姆比克斯·莫里丝腺对于丝生产至关重要.
化使B. mori对诸如Cyantraniliprole (Cya) 这样的杀虫剂敏感.

研究的目的:

调查Cya毒性对B. mori丝腺发育的影响.
为了阐明Cya诱导的丝腺损伤背后的分子机制.

主要方法:

第五阶段B. mori幼虫暴露于不同度的Cya (LC5,LC10,LC20).
评估物理参数 (体重,丝腺指数,子质量).
脊髓病理学,超结构和转录组测序分析后部丝腺 (PSG).
定量实时PCR (qRT-PCR) 用于基因表达分析.

主要成果:

暴露 (LC10,LC20) 显著降低了体重,丝腺体重,丝腺指数和子质量,以剂量依赖的方式.
组织病理学揭示了核膜和内质网膜的破坏,导致PSG细胞中真空球的形成.
转录组分析发现了2152个差异表达的基因,蛋白质合成相关途径 (主要是下调) 显著丰富.
在PSG细胞中,Cya LC10破坏了DNA复制,蛋白质加工,运输和水解.

结论:

短期暴露于Cya会对B. mori丝腺的发育和功能产生负面影响.
毒性破坏了关键的细胞过程,包括丝腺中的DNA复制和蛋白质代谢.
这些发现为在乳养殖中安全使用西安特拉尼利普罗尔提供了基础.

关键词:

波姆比克斯 (Bombyx mori mori mori) 是一个有趣的动物.兰利烯 (Cyantraniliprole) 是一种兰利烯.细胞内膜网膜的内细胞网膜.后面的丝腺腺体蛋白质合成蛋白质合成

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