脱矿性牙基质水凝的生物相容性和抗菌作用,用于牙纤维的保存
Nessma Sultan1,2, Josette Camilleri3, Ben A Scheven4
1Department of Oral Biology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt. nesmasultan88@gmail.com.
Odontology
|September 14, 2024
概括
一种新的生物模拟性纸封闭剂,DDMH,促进牙纸干细胞的活力和原蛋白的产生. DDMH显示出作为再生性内牙材料的潜力,在体外表现优于MTA.
科学领域:
- 生物材料科学 生物材料科学
- 恢复性牙科 恢复性牙科
- 牙纸生物学 牙纸生物学
背景情况:
- 生命脉疗法旨在再生牙并保持脉活力.
- 需要新的仿生材料来增强牙再生活动.
- 脱矿牙基质 (DDM) 颗粒为再生提供了一个有前途的基板.
研究的目的:
- 为了评估一个新的生物仿真纸封闭剂,DDMH,其再生潜力.
- 在体外比较DDMH与矿物三氧化物聚合物 (MTA) 的疗效.
- 评估DDMH对牙髓干细胞 (DPSC) 活力,增殖和基因表达的影响.
主要方法:
- 使用去矿化牙基质 (DDM) 颗粒和酸合成了DDMH.
- 用MTT测定对暴露于DDMH和MTA化剂的DPSC进行了细胞毒性评估.
- 通过实时定量PCR分析了原-I (COL-I) 基因表达.
- 细胞生长,附着和抗菌活性使用阿克里丁色染色和阿加扩散试验进行了评估.
主要成果:
- DDMH化剂促进了DPSC的活力,而MTA化剂在72小时后表现出细胞毒性.
- 与MTA不同的是,DPSC在DDMH上表现出强的增长和依恋,而不是在MTA上.
- 与MTA相比,在用DDMH化剂培养的DPSC中,COL-I基因表达显著上调.
- 在24小时后,DDMH对对照和MTA表现出显著的抗菌活性.
结论:
- 与MTA相比,DDMH在体外显示出更高的生物相容性和再生潜力.
- DDMH促进了DPSC的增殖,分化 (COL-I表达),并具有抗菌性质.
- 在再生性内牙科中,DDMH代表了一种有前途的替代性纸封闭剂.
相关概念视频
Waterproofing and Anti-Bacterial Admixtures in Concrete
329
Concrete's susceptibility to water absorption is due to the capillary action within the pores of its hydrated cement paste. This action draws water in, creating the need for waterproofing admixtures to prevent such penetration. The efficacy of these admixtures is contingent upon the water pressure, with variations arising from different conditions such as rain, capillary rise, or hydrostatic pressure in structures intended to hold water.
Waterproofing admixtures render concrete hydrophobic,...
Waterproofing admixtures render concrete hydrophobic,...
329
Microorganisms in Agriculture and Food industry
2.0K
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
2.0K
Biofilms
2.1K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
2.1K
The Oral Microbiota
90
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
90


