相关实验视频

Updated: Jun 13, 2025

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
10:39

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Published on: September 14, 2014

29.8K

在亚纳米分辨率下蛋白质的低剂量冷电子图解

Berk Küçükoğlu1, Inayathulla Mohammed1, Ricardo C Guerrero-Ferreira1,2

  • 1Laboratory of Biological Electron Microscopy, Institute of Physics, School of Basic Sciences, EPFL, and Department of Fundamental Microbiology, Faculty of Biology and Medicine, UNIL, Rte. de la Sorge, 1015, Lausanne, Switzerland.

Nature communications
|September 14, 2024
PubMed
概括

使用4D扫描传输电子显微镜 (4D-STEM) 的电子图谱提高了冷EM的信号噪声比. 这项技术实现了冷水合蛋白质的亚纳米分辨率3D重建.

更多相关视频

Electron Cryotomography of Bacterial Cells14:23

Electron Cryotomography of Bacterial Cells

Published on: May 6, 2010

25.4K
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy09:16

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Published on: February 7, 2022

6.2K

相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K