来自巴基斯坦的耐莱涅佐利德耐药Enterococcus faecium临床分离物:基因组分析
Syed Ali Raza Nasir1, Mohammad Zeeshan1, Najia Ghanchi1,2
1Aga Khan University Hospital, Karachi, Sindh, Pakistan.
BMC microbiology
|September 14, 2024
概括
巴基斯坦的Linezolid耐药Enterococcus faecium (LRE) 菌株主要是CC17,携带耐药基因,如 optrA, poxtA 和 cfr(D). 这些发现突显了全球重要病原体中新兴的耐药机制.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 耐莱涅佐利德的Enterococcus faecium (LRE) 是一个关键的全球病原体.
- 在2021年11月至2023年4月期间,在巴基斯坦卡拉奇发现了13个LRE隔离体.
- 研究菌株类型和抵抗机制对于了解LRE传播至关重要.
研究的目的:
- 来自巴基斯坦的LRE分离物的菌株类型的特征.
- 确定在这些分离物中赋予线化物耐药性的分子机制.
- 为该地区LRE流行病学提供见解.
主要方法:
- 全基因组测序 (WGS) 用于全面的基因组分析.
- 多部位序列类型 (MLST) 用于确定克隆复合体和序列类型.
- ResFinder和LRE-finder工具发现了特定的linezolid耐药基因和突变.
主要成果:
- 在13个LRE分离物中,12个属于克隆复合体17 (CC17),包括ST80,ST612和ST1380.
- 六个分离物含有23S rRNA基因中的 optrA基因和 G2576T突变.
- 六个分离物携带poxtA和cfr(D) 基因,其中一个分离物表现出所有三种耐药性决定因素.
结论:
- 该研究证实了CC17序列类型在巴基斯坦的流行,这些类型以其爆发潜力而闻名.
- 确定了对线化物耐药性的新型分子机制,包括 optrA,cfr(D 和 poxtA 的组合.
- 这些发现代表了来自巴基斯坦的LRE中这些特定抵抗机制的首份报告.
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