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在各种营养介质上分离的Mycobacterium abscessus复杂菌株质谱的比较分析

Ekaterina Vasilievna Vyzhigina¹, Alexander Mikhailovich Kovalyov², Daniil Andreevich Kokorev²

¹Department of Phthisiology and Pulmonology, Samara State Medical University, Samara, Russia.

International journal of mycobacteriology

|September 15, 2024

概括

为Mycobacterium abscessus complex (MABSc) 隔离优化营养介质可以改善质谱识别,这对于囊性纤维化患者至关重要. 为了准确地确定MABSc.的亚种,需要进一步细化.

科学领域:

临床微生物学临床微生物学
传染病传染病传染病
在诊断中使用质谱仪.

背景情况:

菌根综合体 (MABSc) 导致慢性呼吸道感染,特别是在囊性纤维化患者中.
准确识别MABSc对于有效治疗至关重要,质谱仪 (MS) 提供了速度和成本效益.
目前的MS方法需要优化,以便可靠地识别MABSc.等非结核性菌根菌的亚种.

研究的目的:

评估不同营养介质对基于质谱的MABSc菌株鉴定的影响.
评估MALDI-ToF MS的准确性,用于MABSc亚种的识别.
确定种植和样本准备的潜在改进,以加强MABSc识别.

主要方法:

从囊性纤维化和其他肺病患者的64个MABSc菌株在通用染色体和选择性介质上培养.
使用矩阵激活激光飞行时间脱落/电离质谱法 (MALDI-ToF MS) 进行了物种鉴定.
亚特异性识别使用了rpoB和hsp65基因片段的测序.

主要成果:

在不同介质上培养的MABSc菌株之间观察到质谱学概况的显著差异 (P < 0.001).
用MALDI-ToF MS在通用染色介质上进行的亚种识别显示,与15个菌株的测序结果一致86.6%.
MALDI-ToF MS为MABSc提供了快速和具有成本效益的微生物识别,但有限的亚种解决方案.

结论:

营养介质的选择影响了MALDI-ToF MS.的MABSc识别.
MALDI-ToF MS是快速识别MABSc的宝贵工具,但需要进一步优化以获得亚种级准确性.
为了更好地识别MABSc亚种,需要改进种植技术和蛋白质提取.

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