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Updated: Jun 13, 2025

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

基于肠道益生菌的纳米颗粒用于细胞毒性siRNA输送,用于抗癌免疫疗法

Manfang Zhu¹, Xiaohua Chen², Yueyang Zhang¹

¹Department of Pharmacy, Personalized Drug Therapy Key Laboratory of Sichuan Province Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China.

International journal of pharmaceutics

|September 15, 2024

概括

这项研究引入了一种基于Lactobacillus reuteri的新平台,用于癌症免疫疗法. 双功能载体增强免疫反应,并提供siRNA以有效抑制瘤.

科学领域:

在瘤学瘤学.
免疫治疗是一种免疫疗法.
基因治疗基因治疗

背景情况:

免疫基因疗法将基因疗法与免疫刺激结合起来,用于癌症治疗.
现有的平台往往难以平衡免疫刺激和高效的基因传递.
乳杆菌reuteri表现出与癌症进展相关的免疫调节特性.

研究的目的:

为癌症免疫基因疗法开发一个理想的平台,实现免疫刺激和高效的基因传递.
为了研究 Lactobacillus reuteri lysate 作为一种新型非病毒 siRNA 载体的组成部分的潜力.
评估免疫激活和Stat3沉默用于瘤抑制的联合策略.

主要方法:

设计了一种新的非病毒性siRNA载体 (DMPLAC),通过在DMP中封装Lactobacillus reuteri lysate.
评估DMPLAC促进免疫细胞成熟和激活的能力.
研究了瘤微环境中抗原呈现细胞 (APC) 和细胞毒性T细胞的透.
装载DMPLAC与siRNA向Stat3 (DMPLAC/siStat3) 进行增强瘤抑制.

主要成果:

DMPLAC有效地促进了免疫细胞的成熟和激活.
在瘤微环境中观察到APC和细胞毒性T细胞的透增加.
在多个模型中,DMPLAC/siStat3表现出显著的瘤抑制作用.
双功能设计成功地将免疫激活与Stat3沉默相结合.

结论:

新的DMPLAC载体,利用Lactobacillus reuteri lysate,为癌症免疫基因治疗提供了一个有前途的平台.
这种策略有效地触发免疫反应,并通过联合免疫激活和Stat3沉默实现瘤杀伤.
双功能设计为有效的癌症治疗提供了一条新的治疗途径.

关键词:

免疫原疗法是一种免疫性疗法.肠道益生菌是一种益生菌.统计统计统计 siRNA的传递方式

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相关概念视频

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...