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表达模式和功能分析的基因素-14KIFC1在精子生成的澳门mulatta

Ya-Lan Wei¹, Xiao-Jing Fan², Xin-Chen Lin²

¹Medical Research Center, Fujian Maternity and Child Health Hospital, Fuzhou, Fujian 350001, China; College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, Fujian 350122, China; Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fujian Maternity and Child Health Hospital, Fuzhou, Fujian 350001, China.

|September 15, 2024

概括

基因素电机KIFC1在灵长类精子发育中起着关键作用,调节精子的结构和功能. 这项研究揭示了新的KIFC1变异及其在 rhesus子中至关重要的核和体局部.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

在各种物种中,C端激素电机KIFC1对于生殖细胞发育至关重要.
在精子生成过程中,KIFC1调节染色体分离,体运输和核形态.
它在非人类灵长类动物,特别是 rhesus 子中的作用在很大程度上是未知的.

研究的目的:

研究非人类灵长类动物精子生成中的KIFC1的功能和表达.
识别新的KIFC1转录及其功能域.
阐明KIFC1在 rhesus子精子发育和完整性中的作用.

主要方法:

构建一个丸特定的cDNA库.
克隆和KIFC1转录的表征.
在 rhesus子丸中对KIFC1蛋白的时空表达分析.
在 rhesus子精子和 LLC-MK2 细胞中进行局部化研究.
功能性抑制KIFC1,以评估其对精子的影响.

主要成果:

识别具有独特功能域的新KIFC1变体.
KIFC1蛋白在精子细胞和早期精子细胞中表现出核定位.
KIFC1转录在 rhesus LLC-MK2 细胞的核中得到表达.
KIFC1局部存在于成熟精子的青体和鞭毛体中.
抑制KIFC1导致精子尾部变形和头尾连接不稳定.

结论:

这项研究描述了 rhesus 子中的 KIFC1 表达和功能,扩大了对其在灵长类繁殖中的作用的了解.
新的KIFC1变体有助于KIFC1功能在生殖细胞发育中的多样性.
在非人类灵长类动物中,KIFC1对于维持正常的精子形态和结构完整性至关重要.

关键词:

形成一个宏的形成.一个KIFC1一个KIFC1 介质变化 (Meiosis) 是一个过程.精子发生是精子生成.尾部维护尾部维护尾部维护尾部维护

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