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Hsa_circ_0087784通过miR-576-5p/CDCA4轴增强非小细胞肺癌的进展

Bin Shang¹, Long Li¹, Gang Wang¹

¹Department of Cardio-Thoracic Surgery, The Second Affiliated Hospital of Bengbu Medical University, #633 Longhua Road, Huaishang District, Bengbu 233000 Anhui, China.

The American journal of the medical sciences

|September 15, 2024

概括

下调循环RNA hsa_circ_0087784 抑制非小细胞肺癌 (NSCLC) 的进展. 这通过调节CDCA4表达和海绵化miR-576-5p来发生,抑制转移和扩散.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

循环RNAs (circRNAs) 与癌症进展有关.
已发现Hsa_circ_0087784在乳腺癌中异常表达.
在非小细胞肺癌 (NSCLC) 中hsa_circ_0087784的作用仍然未被探索.

研究的目的:

研究hsa_circ_0087784在NSCLC中的表达和功能.
阐明hsa_circ_0087784在NSCLC进展中的作用背后的分子机制.

主要方法:

使用定量实时PCR (RT-qPCR) 和光在位杂交 (FISH) 来评估hsa_circ_0087784表达.
双路西法酶记者测定确定了下游目标.
在体外 (Transwell,EDU,CCK-8) 和体内 (老鼠异种移植) 试验中评估了迁移,增殖,瘤发生和转移.

主要成果:

Hsa_circ_0087784在NSCLC组织和细胞系中显著上调.
下调hsa_circ_0087784抑制了NSCLC细胞的增殖和迁移.
确定了miR-576-5p和CDCA4作为下游目标;CDCA4过度表达或miR-576-5p抑制逆转了hsa_circ_0087784沉默的影响.

结论:

下调hsa_circ_0087784抑制NSCLC的转移和进展.
这种抑制通过调节CDCA4表达和miR-576-5p的海绵作用来调节.

关键词:

这是一个CDCA4CDCA4CDCA4CDCA4CDCA.皮质 - 介质细胞过渡 (EMT)这是一个很棒的平台.非小细胞肺癌 (NSCLC) 是一种非小细胞肺癌.在 miR-576-5p 中使用.

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