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在淡水双鱼Hyriopsis cumingii中,PPAR和CPT1之间的分子表征和转录调节

Yu Sun¹, Jie Li¹, Yanhong Li¹

¹College of Fisheries, Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Research Center of Fishery Resources and Environment, Southwest University, Chongqing 400715, China.

International journal of biological macromolecules

|September 15, 2024

概括

在软体动物中研究过氧酶增殖器激活受体 (PPAR) 和卡尼丁棕转移酶1 (CPT1) 信号通路,这对于脂质代谢和免疫是至关重要的. 研究人员发现证据表明,这种途径存在于Hyriopsis cumingii中,影响先天免疫力.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
海洋生物学海洋生物学

背景情况:

过氧体增殖器激活受体 (PPARs) 通过卡尼丁棕基转移酶1 (CPT1) 交换激活来调节脂质代谢和适应性免疫.
软体动物中PPAR-CPT1信号通路的存在,这些软体动物只具有先天免疫力,仍然未被探索.

研究的目的:

克隆和描述软体动物Hyriopsis cumingii中的PPAR和CPT1基因.
调查软体动物先天免疫和脂质代谢中PPAR-CPT1信号通路的潜在存在和功能.

主要方法:

来自Hyriopsis cumingii的HcPPAR和HcCPT1的基因克隆和生物信息学分析.
实时定量PCR用于评估不同组织中的基因表达水平.
对抗剂/抑制剂治疗和双 luciferase 记者测定,以确定路径相关性和调节机制.

主要成果:

在物种之间确定了HcPPARs和HcCPT1s的保存分子特征.
在血液,地幔和肠道中HcPPARs和HcCPT1s的更高表达表明它们在软体动物脂质代谢和先天免疫力中的作用.
证据表明HcPPARs和HcCPT1s表达之间的相关性,在HcCPT1s促进器上确定了潜在的HcPPARs结合位点.

结论:

在Hyriopsis cumingii中可能存在PPAR-CPT1信号通路.
这项研究为PPAR-CPT1信号在软体动物天生的免疫力中的作用提供了基础证据.
研究结果提供了有关软体动物分子调节机制的见解,以及对代谢和炎症疾病的潜在治疗点.

关键词:

这是天生的免疫力.脂质新陈代谢脂质新陈代谢在PPAR-CPT1信号传输中.

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