基于银丝尼达纳米混合乳液的益生菌作为一种新型抗真菌配方,用于对抗多药耐药性阴道病原体
Farag M Mosallam1, Eman A Helmy2, Hanan S El-Bastawisy1
1Division of Biotechnology, Microbiology Lab, Department of Drug Radiation Research, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.
Biotechnology and applied biochemistry
|September 16, 2024
概括
一种新的基于银色秒尼达纳米混合乳液 (Ag-Secn-NHE) 的益生菌有效地对抗多药耐药性阴道感染. 这种创新方法显示出优越的抗真菌活性和生物膜抑制对常见的病原体,如疹.
科学领域:
- 菌类学和制药科学 菌类学和制药科学
- 纳米技术在医学中的应用
- 抗微生物耐药性研究研究
背景情况:
- 阴道感染,特别是角膜炎,对健康构成重大挑战,通常涉及多种耐药性病原体.
- 现有治疗方法在有效性和耐药性发展方面可能存在局限性.
- 新的治疗策略对于治疗持续性和耐药性阴道感染至关重要.
研究的目的:
- 开发和评估一种新的基于银色二二醇纳米混合乳液 (Ag-Secn-NHE) 的益生菌配方,用于管理阴道角膜病.
- 评估基于Ag-Secn-NHE的益生菌对关键的阴道病原体的抗真菌疗效和作用机制.
- 将新药配方的性能与现有治疗方法及其组件进行比较.
主要方法:
- 使用同质化-超声波和通过UV-Vis,DLS,TEM,FTIR和zeta潜力进行表征的Ag-Secn-NHE的制备.
- 将Ag-Secn-NHE与Saccharomyces cerevisiae (RCMB 002Y001) 结合在一起,以创建益生菌配方.
- 抗真菌敏感性测试 (MIC,MFC),生物膜抑制测定,时间杀伤动力学和传输电子显微镜 (TEM) 进行超结构分析.
主要成果:
- 与免费的Ag-Secn-NHE和其他对照剂相比,基于Ag-Secn-NHE的益生菌显著增强了对Candida auris,Candida albicans和Cryptococcus neoformans的抗真菌作用.
- 该配方实现了强大的最小抑制度 (MIC) 和最小杀菌度 (MFC) 对测试的病原体.
- 观察到显著的生物膜形成抑制 (高达75%),TEM揭示了治疗后Candida auris细胞的严重超结构损伤.
结论:
- 开发的基于Ag-Secn-NHE的益生菌代表了一种有前途的新型治疗剂,用于对抗多药耐药性阴道感染.
- 益生菌和纳米混合乳液的协同组合增强了抗真菌功效和生物膜破坏.
- 需要进一步的研究和临床试验,以探索这种创新的配方的全部治疗潜力.
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