通过TGF-β信号通路调节在CRC中原生益生菌乳杆菌物种的作用
Amin Sepehr1, Shadi Aghamohammad1, Roya Ghanavati2
1Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Iranian biomedical journal
|September 16, 2024
概括
一种新的Lactobacillus混合物显著抑制了结直肠癌 (CRC) 细胞增殖,并在体外和体外模型中降低了转化生长因子β (TGF-β) 途径中的关键基因.
科学领域:
- 微生物学和癌症研究研究
- 肠道微生物组和结直肠癌 (CRC) 的相互作用
- 益生菌对癌症信号通路的影响.
背景情况:
- 结肠直肠癌 (CRC) 与结肠微生物组合的显著变化有关.
- 转化生长因子β (TGF-β) 信号通路在CRC的发展和进展中起着至关重要的作用.
- 研究益生菌对CRC病原体的影响是一个有前途的研究领域.
研究的目的:
- 评估Lactobacillus混合物对结直肠癌 (CRC) 细胞的抗增殖作用.
- 为了评估这种Lactobacillus混合物对转化生长因子β (TGF-β) 信号通路的影响.
- 在体外和体外模型中确定Lactobacillus混合物在调节CRC进展方面的疗效.
主要方法:
- 实验室抗增殖试验 (MTT) 在用乳杆菌混合物治疗的HT-29癌细胞上进行.
- 量化逆转录PCR (qRT-PCR) 用于分析TGF-β通路组件 (受体,调控和抑制SMAD) 的基因表达水平.
- 在体内研究包括在BALB/c小鼠中诱导的氧甲/德克斯硫酸盐 (AOM/DSS) 诱导的CRC,其中小组接受了PBS或Lactobacillus混合物.
主要成果:
- 乳酸菌混合物在治疗120小时后显著降低HT-29癌细胞的增殖.
- 基因表达分析显示,在接受治疗的癌细胞中,smad2/3和smad4的下调.
- 在体内,Lactobacillus混合物降低了AOM/DSS诱导的CRC小鼠模型中的tgf-βR1和smad4表达.
结论:
- 新型益生菌Lactobacillus混合物对结直肠癌 (CRC) 细胞表现出显著的抗增殖作用.
- 观察到的抗癌效应与转化生长因子β (TGF-β) 信号通路的下调有关.
- 这种Lactobacillus混合物显示出作为抑制CRC进展的治疗剂的潜力.
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