相关实验视频
Updated: Jul 19, 2026
05:44
In vitro Cell Culture Model for Toxic Inhaled Chemical Testing
Published on: May 8, 2014
10.7K
两种不同度的甲酸盐的细胞毒性作用:一个体外研究
Divya Mukundan1, Ganesh Jeevanandan2
1Pediatric and Preventive Dentistry, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Cureus
|September 16, 2024
概括
儿童牙医应使用较低度的二酸盐 (NaOCl) 进行一次性牙内灌. 1%的NaOCl溶液对盐水的毒性明显低于3%的NaOCl溶液.
科学领域:
- 牙科 牙科是指牙科的专业.
- 毒理学 毒理学 毒理学
- 生物医学科学 生物医学科学
背景情况:
- 由于独特的解剖学,初级牙的内牙治疗是复杂的.
- 低化 (NaOCl) 是一种常见的灌剂,但较高度可能对周围组织有毒.
- 选择合适的NaOCl度对于儿科内科医生至关重要.
研究的目的:
- 为了评估和比较1%和3%的二 (NaOCl) 溶液在不同体积的细胞毒性.
- 为了确定用于母牙内灌的NaOCl的安全度.
主要方法:
- 用盐水 (nauplii) 致死性试验评估了细胞毒性.
- 纳乌普利培养被暴露在5%和3%的NaOCl溶液中的5,10,20和40μL中.
- 盐水作为对照组,并在24小时后记录了nauplii生存率.
主要成果:
- 对照组没有显示出婴儿死亡率.
- 1%的NaOCl在测试卷中导致10-20%的死亡率,没有显著差异 (p=0.193).
- 3%的NaOCl导致的死亡率明显更高,从20%到60% (p=0.007),其中40μL是最有毒的.
结论:
- 与3%的NaOCl溶液相比,1%的甲基化物 (NaOCl) 溶液的细胞毒性明显降低.
- 在儿科牙科中,建议使用较低的NaOCl度进行内牙灌,以最大限度地减少周围组织的毒性.
- 这些发现支持儿童更安全的内牙治疗结果.
相关概念视频
01:12Bacterial Toxins
Bacterial toxins are sophisticated virulence factors that enable pathogenic bacteria to interact with, invade, and damage host tissues. These toxins fall broadly into two types: protein exotoxins, which are secreted into the environment and target specific host receptors, and lipopolysaccharide endotoxins, which are structural components of the bacterial outer membrane released primarily during bacterial lysis or membrane shedding. Exotoxins generally act more selectively, binding to cell...
01:20Determinants of Bacterial Pathogenicity and Virulence
Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...