来自Penicillium aurantiancobrunneum的类固醇和皮
Charmaine A Lindsay1, Choon Y Tan1, Deepa Krishnan2
1Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
Phytochemistry letters
|September 16, 2024
概括
从与相关的真菌Penicillium aurantiacobrunneum中分离出了新的类固醇. 化合物1对胰腺癌细胞表现出细胞毒性活性,这表明有可能发现新药.
科学领域:
- 自然产品化学 自然产品化学
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 类动物的宿舍是产生生物活性二次代谢物的共生真菌 (mycobionts).
- 与菌相关的真菌是发现新型细胞毒性化合物的有希望的来源.
- 研究人员对Penicillium aurantiacobrunneum,一种从中分离出来的真菌进行了研究,以确定其化学成分.
研究的目的:
- 从Penicillium aurantiacobrunneum中分离和描述新的化合物.
- 评估分离化合物的细胞毒性潜力.
- 为已识别的化合物提出一种生物合成途径.
主要方法:
- 培养的 青虫 aurantiacobrunneum. 的种植.
- 使用色谱技术分离和净化二次代谢产物.
- 通过核磁共振 (NMR) 光谱和高分辨率电喷射电离化质谱法 (HRESIMS) 阐明结构.
- 使用HPAC细胞系进行细胞毒性测定.
主要成果:
- 已经分离出四种新的固醇 ((20S*) - 基-24(28) - 脱卡斯特醇 (1),7α-甲基-8β-基巴斯特醇 (2),14-nor-epicoccarine A (3),14-nor-epicoccarine B (4)) 和一种已知的化合物 (PF1140 (5)).
- 化合物1对HPAC细胞系表现出细胞毒性,其IC50值为17.76 ± 5.35μM.
- 化合物3和4与四酸衍生物具有结构上的相似性,这表明PKS-NRPS混合生物合成起源.
结论:
- 青 (Penicillium aurantiacobrunneum) 是一种具有潜在细胞毒性活性的新型类固醇的来源.
- 化合物1代表了开发新的胰腺癌治疗方法的领先地位.
- 提出了涉及PKS-NRPS混合系统的化合物3和4的可信生物合成途径.
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