酸积极调节玉米对抗类昆虫的防御能力
Yohannes Besufekad Setotaw1,2, Jing Li1,2, Jinfeng Qi1,2
1Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Plant diversity
|September 16, 2024
概括
酸 (SA) 通过控制乙烯信号和防御代谢物,积极调节玉米对昆虫食草的防御. 阻止玉米植物中的SA积累减少了昆虫的抵抗力.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 农业 农业 农业 农业
背景情况:
- 植物利用植物激素信号通路,包括莉酸 (JA),乙烯 (ET) 和酸 (SA),以防御昆虫的攻击.
- 虽然已知JA和ET在玉米防御中的作用,但SA在玉米对昆虫食草动物的抵抗中的作用尚不清楚.
研究的目的:
- 研究酸 (SA) 在调节玉米对昆虫食草动物的防御中的作用.
- 阐明在玉米中SA介导植物防御的信号通路和分子机制.
主要方法:
- 在玉米中NahG (酸盐氧酶) 基因的异胎表达,以阻止SA积累.
- 与野生型 (WT) 玉米相比,评估NahG玉米对通用型和专业型虫幼虫的昆虫耐药性.
- 在WT和NahG玉米中量化植物激素水平 (JA,JA-Ile,ET) 和防御代谢物 (氧化物,酸).
- 转录组分析以确定潜在的监管因素.
主要成果:
- 与WT玉米相比,NahG玉米对Spodoptera litura,Spodoptera frugiperda和Mythimna separata的耐药性降低了.与WT玉米相比,NahG玉米的耐药性降低了.
- 在NahG玉米中受损的耐药性与本佐酸和酸水平的降低有关.
- SA积极调节ET信号传输,但不会影响JA或JA-单离子素合物水平.
- 证据表明,在调节防御性代谢物积累方面,SA,JA和ET途径之间没有交叉通话.
结论:
- 酸 (SA) 在增强玉米对虫幼虫的防御方面发挥着至关重要的作用.
- SA积极调节乙烯信号,有助于植物的耐药性.
- 这项研究发现了一种涉及到玉米昆虫防御的新型植物激素途径,突出了作物保护的潜在目标.
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