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在人类正常肝细胞模型中,环境诱导的circRNA-miRNA-mRNA网络调节机制

Zhi Qu^1,2, Lugang Deng^1,3, Chunqian Feng⁴

¹Institute of Environment and Health, South China Hospital of Shenzhen University, Shenzhen 518116, China.

Current research in toxicology

|September 16, 2024

概括

循环RNAs (circRNAs) 在 (Cd) 毒性中发挥作用,通过调节肝细胞过程,如亡和氧化应激. 这项研究揭示了circRNAs作为Cd毒性机制的关键参与者.

科学领域:

分子生物学分子生物学
毒理学毒理学毒理学
基因组学就是基因组学.

背景情况:

数百种长的非编码RNA (lncRNA) 和微RNA (miRNA) 与 (Cd) 毒性有关.
循环RNAs (circRNAs) 在Cd毒性中的作用及其调节机制尚不清楚.

研究的目的:

使用人类正常肝细胞 (L-02) 调查circRNAs在Cd毒性的作用.
阐明Cd诱导的毒性背后的调节机制,涉及circRNA,miRNA和信使RNA (mRNA).

主要方法:

在暴露于Cd.的L-02细胞中的circRNAs,miRNAs和mRNAs的全转录组表达概况.
对差异表达的circRNAs,miRNAs和mRNAs进行鉴定.
构建一个竞争的内源RNA (ceRNA) 网络.
基因本体学 (GO) 丰富和KEGG通路分析.

主要成果:

鉴定了266个差异表达的环RNA,223个miRNA和519个mRNA.
构建了一个ceRNA网络,涉及6个circRNA,10个miRNA和97个mRNA.
GO和KEGG分析表明,circRNAs调节细胞增殖,细胞亡,氧化应激和炎症反应.

结论:

circRNAs通过调节肝细胞中的关键细胞过程,参与Cd毒性.
已识别的ceRNA网络为Cd诱导的肝损伤的分子机制提供了洞察力.
circRNAs代表了减轻Cd毒性的潜在治疗点.

关键词:

是的组成部分.这就是CeRNA.循环RNA 是一个循环RNA.环境因素环境因素一个L-02细胞.

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