来自混合培养中以部分脱功能细菌占主导地位的EPS分片的表征
Jiapeng Li1,2, Yanxi Chen1,2, Ji Qi1,2
1School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, PR China.
Water research X
|September 16, 2024
概括
部分脱 (PD) 协会中的细胞外聚合物物 (EPS) 丰富且富含蛋白质,结构紧. 这种结构可能会限制细胞聚合,这表明需要优化过程以提高生物反应器的性能.
科学领域:
- 环境微生物学环境微生物学
- 生物技术是生物技术.
- 污水处理 污水处理 污水处理
背景情况:
- 细胞外聚合物物质 (EPS) 对微生物聚合和生物反应器性能至关重要.
- 在部分脱 (PD) 联合体中,EPS的结构和组成特性仍然未被描述.
研究的目的:
- 调查来自PD联盟的EPS的结构和组成特征.
- 为了将EPS特性与污泥聚合潜力和生物反应器性能相关联.
主要方法:
- 用于EPS量化的光度测量.
- 福里埃变换红外光谱 (FTIR) 用于功能组分析.
- 对表面元素组成的X射线光电子光谱 (XPS).
- 对分层EPS分数组 (TB-EPS,LB-EPS,S-EPS) 的分析.
主要成果:
- PD联盟的EPS含量显著高 (168.81 mg/gVSS) 比传统活性污泥 (79.79 mg/gVSS) 高得多.
- PD联盟的EPS显示蛋白质占主导地位 (蛋白质/多糖比为1.43),水友群较少,这表明聚合潜力很高.
- 在PD联盟EPS中的蛋白质具有0.99的紧结构 (高α-螺旋/β-片+随机线圈) 比率),可能阻碍细胞聚合.
结论:
- 来自PD联盟的EPS与传统污泥相比,具有独特的结构和组成特性.
- 在PD联盟EPS中的紧蛋白质结构可能会限制污泥聚合,需要优化工艺.
- 了解PD EPS特征对于增强基于PD的废水处理工艺的应用至关重要.
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