抗微生物耐药性,多位菌序列,和spa类型的金黄色葡萄球菌从零售生肉制品中分离出来
1Faculty of Arts and Sciences, Department of Biology, Bolu Abant Izzet Baysal University, 14030 Gölköy, Bolu, Turkey.
BioMed research international
|September 16, 2024
概括
在零售肉类中发现了多药耐药的金黄色葡萄球菌菌株. 这些携带各种抗性基因的食物传播细菌,由于可能传染给人类,对公共健康构成重大风险.
科学领域:
- 食品微生物学 食品微生物学
- 抗微生物耐药性 抗微生物耐药性
- 分子流行病学分子流行病学
背景情况:
- 黄金葡萄球菌 (Staphylococcus aureus) 是一个重要的病原体,以获得抗菌素耐药性而闻名.
- 肉制品可以作为耐药细菌菌株的储存库.
研究的目的:
- 从零售肉制品中分离出的金黄色葡萄球菌的特征.
- 为了确定抗微生物耐药性概况,SPA类型和序列类型.
- 评估食物链中耐药黄金杆菌对公共卫生的影响.
主要方法:
- 使用微稀释进行抗菌素耐药性分析.
- 检测特定的耐药性基因 (blaZ,sulI,grlA,norA,tetK,chlA,tetM,aacA-aphD,erMA,fexA,dfrA) 的情况.
- 葡萄球菌蛋白A (spa) 基因类型和多部位序列类型 (MLST).
主要成果:
- 所有50个S. aureus分离物都对青素和硫甲醇有抗性.
- 抗性基因 blaZ 和 sulI 的高患病率 (100%),其次是 grlA (94%) 和 norA (92%).
- 26%的分离物是多药耐药的;确定了22种spa类型和7种MLST序列类型 (ST),包括新型类型.
结论:
- 零售肉类港口具有多药耐药的金黄色细菌与与人类感染有关的克隆.
- 动物来源的食物可以传播耐药的金黄色细菌,对健康构成风险.
- 持续监测至关重要,以监测病原性黄金杆菌通过食品供应传播.
相关概念视频
01:29Staphylococcal Skin Infections
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
01:25Mechanism of Antibiotic Resistance in MRSA
Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
01:25Clinical Significance of Antibiotic Resistance
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...