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相关概念视频

RNA Editing02:23

RNA Editing

8.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.9K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

7.5K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.5K
Mismatch Repair01:20

Mismatch Repair

4.8K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.8K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

3.1K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.1K
Base Excision Repair01:54

Base Excision Repair

22.2K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
22.2K

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相关实验视频

Updated: Jun 13, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
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Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

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使用mitoBEs进行有效和链选择性线粒体基编辑的简化过程.

Xiaoxue Zhang1, Zongyi Yi2, Wei Tang2,3

  • 1Changping Laboratory, Beijing 102206, China.

Biophysics reports
|September 16, 2024
PubMed
概括
此摘要是机器生成的。

线粒体基编辑器 (mitoBEs) 为治疗线粒体疾病提供了精确高效的线粒体DNA编辑. 这项研究详细介绍了使用mitoBEs的协议,强调了它们的链选择性和多功能性.

关键词:
脱氨酶是一种脱氨酶.线粒体DNA基组编辑器 (mitoBEs) 的使用尼克·凯斯 (Nickase) 是一个名人.类似转录激活器效应器 (TALE)

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Methodology for Accurate Detection of Mitochondrial DNA Methylation
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Methodology for Accurate Detection of Mitochondrial DNA Methylation

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相关实验视频

Last Updated: Jun 13, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

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Methodology for Accurate Detection of Mitochondrial DNA Methylation
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Methodology for Accurate Detection of Mitochondrial DNA Methylation

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Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights
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Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物技术是生物技术.

背景情况:

  • 线粒体疾病由于难以准线粒体DNA而带来了重大挑战.
  • 现有的基因编辑工具往往缺乏线粒体应用所需的精度和效率.

研究的目的:

  • 为线粒体基编辑器 (mitoBEs) 提供详细的实验协议.
  • 用先进的基础编辑技术促进线粒体疾病的调查和治疗.

主要方法:

  • 转录激活器样效应体 (TALE) 蛋白与单链DNA除氨酶 (例如TadA8e-V106W,APOBEC1) 和尼克酶 (例如MutH,Nt.BspD6I(C)) 的集成.
  • 开发一种可编程的基编辑系统,用于线粒体基因组内的有针对性的修改.

主要成果:

  • 线粒体基编辑工具 (mitoBEs) 在编辑线粒体DNA方面表现出高精度和效率.
  • 该系统提供线程选择性,使得有针对性的基调修改成为可能.
  • 多功能编辑功能是通过使用各种deaminases实现的.

结论:

  • 在研究和治疗线粒体疾病方面,MitoBEs代表了一个有前途的进步.
  • 提供的协议使研究人员能够实现熟练的线粒体基编辑.
  • 这项技术有可能用于针对线粒体DNA缺陷的各种治疗应用.