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在RA患者中,miR-6089可以预防导致心血管疾病的炎症事件
Afsaneh Shamsi1,2, Seyed Askar Roghani1,3,4, Mohammad Shamsi5
1Immunology Department, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Heliyon
|September 16, 2024
概括
在类风湿性关节炎 (RA) 中,长非编码RNA HIX003209 和微RNA miR-6089 与心血管疾病 (CVD) 风险有关. MiR-6089显示出抗炎作用,可能对与RA相关的心血管疾病进行保护,而HIX003209可能会干扰这种保护.
科学领域:
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
- 心脏病学 心脏病学
背景情况:
- 心血管疾病 (CVD) 是类风湿性关节炎 (RA) 患者的主要并发症.
- 失调的非编码RNA表达有助于RA相关的炎症和心血管并发症.
- 长非编码RNA (lncRNA) HIX003209与RA炎症有关.
研究的目的:
- 研究lncRNA HIX003209及其下游微RNA,miR-6089与RA患者的心血管和炎症生物标志物的关联.
- 探索HIX003209和miR-6089在RA相关心血管疾病的发病过程中的潜在作用.
主要方法:
- 招募了60名RA患者 (30名新诊断,30名正在接受治疗) 和30名健康对照.
- 使用实时PCR测量HIX003209,miR-6089和CXCR3的基因表达.
- 使用系统冠状动脉风险评估 (SCORE) 和弗雷明汉风险评分 (FRS) 评估心血管疾病风险.
主要成果:
- 与其他组相比,新诊断的RA患者的LncRNA-HIX003209表达显著增加 (p < 0.05).
- 与对照组相比,在接受治疗的RA患者中,MiR-6089的表达显著增加 (p < 0.001).
- 在LncRNA-HIX003209和CXCR3表达之间发现了正相关性 (p < 0.01).
- 在miR-6089表达和DAS-28,NT-proBNP和CXCL9水平之间发现了负相关性 (p <0.05).
结论:
- MiR-6089可能具有抗炎作用,通过抑制诸如CXCL9和NT-ProBNP之类的炎症化学因子,有可能预防RA中的心血管并发症.
- 增加LncRNA-HIX003209的表达可能会抵消miR-6089在RA患者的保护性抗炎作用.
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