构建状软骨器官体,以探索主要毛的功能
Zhan Liu1, Haoyu Zhou1, Qingwei Wu1
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, PR China.
Heliyon
|September 16, 2024
概括
我们开发了一种状软骨器官模型,可以更好地模仿自然组织结构和细胞外基质. 这种有机体系统对于研究初级毛功能和刺信号通路来说是卓越的.
科学领域:
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
- 生物化学 生化学
背景情况:
- 与二维培养相比,有机体培养系统提供了本地组织结构和细胞矩阵相互作用的优越回顾.
- 初级乳毛在细胞功能和信号传递中起着至关重要的作用,但它们在复杂组织模型中的行为需要进一步调查.
研究的目的:
- 构建一个状软骨器官模型,准确地反映本地组织结构和细胞外矩阵组成.
- 为了研究初级毛和刺信号在发育的状软骨有机体中的作用.
主要方法:
- 构建一个三维的状软骨器官体.
- 在有机体,二维培养和本地软骨之间对初级毛长度和分布进行比较分析.
- 转录和生化分析以评估与毛相关的基因表达和刺信号.
- IFT88进行了淘汰实验,以评估其对关键蛋白质水平的影响.
主要成果:
- 状软骨有机体成功复制了自然状软骨的区域结构和丰富的细胞外基质.
- 器官体中的初级毛表现出与本地软骨相似的长度,与2D培养中的较短的毛不同.
- 在有机体与二维培养中,IFT88敲击不同影响蛋白质水平 (COL-X,TRPV4,刺信号),有机体的表面区域特别增加.
结论:
- 开发出来的状软骨有机体可以作为一个更具生理相关性的模型来研究初级毛.
- 器官系统提供了一个宝贵的平台,用于剖析在软骨生物学中的初级乳毛和相关信号通路的复杂作用.
关键词:
状软骨有机体是什么刺信号通道的信号通道IFT8888 IFT8888 IFT8888 IFT8888 IFT8888 IFT8888 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88 IFT88主要的乳毛是主要的乳毛.有关RNA测序的RNA测序
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