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新型乳杆菌尾酒通过调节BMP信号通路对结直肠癌发展的抑制作用:体外和活体研究.

Amin Sepehr¹, Shadi Aghamohammad¹, Roya Ghanavati²

¹Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.

|September 16, 2024

概括

一种五种菌株的乳酸菌尾酒显著减少了结直肠癌细胞的增殖,并降低了骨形态遗传蛋白 (BMP) 信号通路的调节. 这种益生菌干预表明,通过抑制关键通路基因和β-catenin,有可能预防结直肠癌 (CRC).

关键词:

在 BMP 信号通道中.结肠直肠癌是一种癌症.牛乳杆菌尾酒是什么意思益生菌益生菌益生菌

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科学领域:

微生物学微生物学
胃肠病学胃肠病学
在瘤学瘤学.

背景情况:

结肠直肠癌 (CRC) 仍然是一个重大的健康问题,异常的信号通路有助于其发展.
骨形态遗传蛋白 (BMP) 信号通路在细胞分化和增殖中起着复杂的作用,其失调与各种癌症有关.
益生菌,特别是乳酸菌种,正在越来越多地研究其潜在的健康益处,包括抗癌性质.

研究的目的:

为了研究一个特定的五种菌株的乳酸菌尾酒对结直肠癌 (CRC) 调节的影响.
为了确定乳酸菌尾酒是否会影响骨形态遗传蛋白 (BMP) 信号通路.
评估Lactobacillus尾酒在体外和体内CRC模型中的抗增殖作用.

主要方法:

在实验室中,使用对HT-29细胞的MTT测定来评估抗繁殖效应.
在体内小鼠模型中使用阿佐西米 (AOM) /硫酸德克斯 (DSS) 来模拟CRC条件.
用基因表达分析 (qRT-PCR) 和蛋白质评估 (西式涂抹) 来评估BMP信号通路活性和β-catenin水平.

主要成果:

乳酸菌尾酒在体外显示出显著的,时间依赖的HT-29细胞增殖抑制.
在接受治疗的细胞和AOM/DSS诱导的小鼠模型中观察到BMP信号通路和hes1基因的下调.
在体外和体外实验中都始终发现β-catenin蛋白表达的显著下降.

结论:

五种菌株的乳酸菌尾酒对结直肠癌细胞表现出抗增殖作用.
该机制涉及BMP信号通路和相关基因的下调,包括hes1和β-catenin.
这些发现表明,这种Lactobacillus尾酒充满希望作为结直肠癌 (CRC) 的预防剂.