主要肝癌类器官及其在研究和治疗中的应用
Xiaobin Zhu1, Rajiv Trehan1, Changqing Xie1
1Thoracic and GI Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, USA.
Journal of the National Cancer Center
|September 16, 2024
概括
主要肝癌类器官提供一个3D体外模型,复制瘤微环境 (TME). 临床翻译需要标准化的协议,使得药物测试和新机制的发现速度更快.
科学领域:
- 在瘤学瘤学.
- 生物技术是生物技术.
- 翻译医学是一种翻译医学.
背景情况:
- 原发性肝癌仍然是一个重大的全球健康挑战.
- 目前的研究模型,如二维细胞培养和动物模型,在完全回顾人类瘤微环境 (TME) 方面存在局限性.
- 癌症有机体代表了一个有前途的3D体外培养系统,它模仿瘤结构和TME.
研究的目的:
- 审查和介绍各种方法来从患者瘤组织中获得原发性肝癌器官.
- 总结肝癌类器官的验证技术,包括免疫组织化学和RNA测序.
- 突出肝癌类器官在药物开发和机制学研究中的潜在应用.
主要方法:
- 关于肝癌有机物衍生协议的出版文献的综述.
- 验证技术摘要:用于标记物表达的免疫组织化学,用于突变和变异的RNA测序.
- 讨论共同培养策略,以纳入免疫细胞并增强TME复制.
主要成果:
- 有各种各样的已发表的方法用于产生肝癌器官.
- 免疫组织化学和RNA测序是有机体表征的关键验证方法.
- 与免疫细胞共同培养有机体可以提高TME忠实度,并有助于研究耐药性.
结论:
- 组织加工和培养介质的标准化对于肝癌器官的临床转化至关重要.
- 肝癌有机物促进高通量药物查和发现新型治疗机制.
- 将有机体与动物模型相结合,可以克服当前体外系统的局限性,推动肝癌研究.
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