高强度间歇训练中升的流星类蛋白质通过AMPK/HDAC4通路改善心脏功能.
Yongshun Wang1,2, Jie Yuan1,2, Huadong Liu1,2
1Department of Cardiology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, Guangdong 518020, China.
Genes & diseases
|September 16, 2024
概括
高强度间歇训练 (HIIT) 通过增加流星样蛋白 (Metrnl) 来改善心力衰竭 (HF). 这种蛋白质通过通过AMPK-HDAC4通路改善葡萄糖代谢和线粒体活性来增强心脏功能.
科学领域:
- 心脏病学 心脏病学
- 运动生理学 运动生理学
- 分子生物学分子生物学
背景情况:
- 高强度间歇训练 (HIIT) 在缓解心力衰竭 (HF) 症状方面比中等强度持续有氧训练 (MICT) 有更大的效果.
- 与MICT相比,在HIIT后观察到升高的流星样蛋白 (Metrnl) 水平.
研究的目的:
- 调查Metrnl在心脏功能改善中的作用.
- 为了阐明涉及到Metrnl介导的心脏益处的信号通路.
主要方法:
- 50名HF患者每人接受了MICT和HIIT,随后进行心脏功能和血清Metrnl测量.
- 患有HF的小鼠接受了HIIT,在血液和骨肌中测量了Metrnl. 用基因操纵 (shRNA) 来评估Metrnl在体内的功能.
- 使用质谱法确定了信号通路,并通过生物化学分析证实了这一点. 评估了心肌细胞的葡萄糖代谢和线粒体功能.
主要成果:
- 在HIIT后,HF患者和小鼠均表现出循环中的Metrnl水平增加.
- 梅特林在心肌细胞中激活了AMPK,导致HDAC4的酸化和失活.
- HDAC4无活化增强了葡萄糖载体4型 (GLUT4) 表达和线粒体复合体表达,改善了有氧葡萄糖呼吸和线粒体功能.
结论:
- HIIT增强了骨肌肉的Metrnl生产,它对心脏起作用,以改善功能.
- AMPK-HDAC4信号通路调解了Metrnl对心脏葡萄糖代谢和HF中的线粒体健康的有益影响.
- 梅特林在中介HIIT在心力衰竭中的心脏保护作用方面发挥着至关重要的作用.
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