基于细胞的疗法可以逆转心力衰竭改变的右心室蛋白质组
Nour Makkaoui1, Vidhya Prasad1, Pritha Bagchi1
1Emory University School of Medicine.
Research square
|September 16, 2024
概括
在压力过载模型中,介质细胞 stromal 细胞 (MSC) 和特定 c-kit 阳性细胞 (CPC) 部分逆转了疾病改变的心脏蛋白质. 这表明细胞治疗有望通过恢复心脏蛋白质组平衡来改善右室功能.
科学领域:
- 心血管生物学 心血管生物学
- 再生医学是一种再生医学.
- 蛋白质组学是指蛋白质组学.
背景情况:
- 先天性心脏缺陷会导致右心室 (RV) 压力过载和心力衰竭.
- 基于细胞的疗法,包括介质干细胞 (MSC) 和c-kit阳性细胞 (CPC),正在探索恢复心脏功能.
- 据信,这些细胞通过膜机制起作用,防止细胞亡并调节细胞功能.
研究的目的:
- 研究患病心脏对不同基于细胞的疗法的蛋白质反应.
- 为了比较MSCs和各种CPC群体在逆转RV压力过载引起的蛋白质变化的有效性.
主要方法:
- 使用肺动脉带带 (PAB) 建立了RV压力过载的动物模型.
- 在RV的自由壁中注射了介质细胞 (MSC) 和三种类型的CPC (nCPC,cCPC,ES-CPC).
- 在RV组织上使用液体染色学和双重质谱进行了蛋白质组分析,随后进行了系统生物学和生物信息学分析.
主要成果:
- 肺动脉带纹显著改变了RV蛋白质组,影响5.97%的已识别的蛋白质.
- 介质细胞治疗逆转了PAB改变的蛋白质组的72%,其次是nCPCs (54%),ES-CPCs (52%) 和cCPCs (39%).
- 细胞疗法显示预测的不良心脏效应减少,并且对未改变的RV蛋白质组影响最小.
结论:
- 介酶体 stromal 细胞,ES-CPCs 和 nCPCs 有效地逆转了 RV 中的压力过载诱导的蛋白质变化.
- 基于细胞的疗法显示了通过部分恢复心脏蛋白质组来改善RV功能后压力过载的显著潜力.
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