相关实验视频

Updated: Jun 13, 2025

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

1.9K

由Piezo1进行的机械感知通过牙周炎的PP2A-Akt轴调节骨质细胞分化

Satoru Shindo, Shin Nakamura, Mohamad Rawas-Qalaji

    bioRxiv : the preprint server for biology
    |September 16, 2024
    PubMed
    概括

    通过前骨质细胞 (前OCs) 上的Piezo1通道进行机械刺激,通过PP2A/Akt通路降低NFATc1的调节,从而抑制骨质再吸收. 这种由Piezo1介导的效应在牙周炎中减少,这表明了治疗潜力.

    更多相关视频

    Less-Invasive Technique for Non-stabilized Mandibular Fracture in Mouse Models04:13

    Less-Invasive Technique for Non-stabilized Mandibular Fracture in Mouse Models

    Published on: September 27, 2024

    312
    A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro07:03

    A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

    Published on: June 16, 2022

    6.0K

    相关概念视频

    Mechanically-gated Ion Channels01:12

    Mechanically-gated Ion Channels

    Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
    6.3K
    Role of Matrix Metalloproteases in Degradation of ECM01:23

    Role of Matrix Metalloproteases in Degradation of ECM

    Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
    2.3K
    Osteoclasts in Bone Remodeling01:31

    Osteoclasts in Bone Remodeling

    Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
    2.8K
    PI3K/mTOR/AKT Signaling Pathway01:22

    PI3K/mTOR/AKT Signaling Pathway

    The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
    3.4K
    Cell-matrix's Response to Mechanical Forces01:13

    Cell-matrix's Response to Mechanical Forces

    In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
    Anchoring junctions mechanically attach a cell to the...
    2.6K
    Tension Response at Adherens Junctions01:26

    Tension Response at Adherens Junctions

    The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
    α-Catenin as a Mechanosensory Protein
    The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
    2.6K