Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Protein Networks02:26

Protein Networks

3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Hyperactive GNAQ in endothelial cells causes blood vessel malformation in developing mouse skin.

Development (Cambridge, England)·2026
Same author

The Trifecta of Polo-Like Kinases, Cancer, and the Immune System: Emerging Intersections and Therapeutic Insights.

Molecular cancer research : MCR·2026
Same author

Development of Potent and Selective RIPK1 Degraders Targeting Its Nonenzymatic Function for Cancer Treatment.

Journal of medicinal chemistry·2025
Same author

Technical Considerations for Detecting Protein-Protein Interactions Using Proximity Ligation Assay.

Journal of proteome research·2025
Same author

Overactive Wnt5a signaling disrupts hair follicle polarity during mouse skin development.

Development (Cambridge, England)·2022
Same author

Functional redundancy of frizzled 3 and frizzled 6 in planar cell polarity control of mouse hair follicles.

Development (Cambridge, England)·2018

相关实验视频

Updated: Jun 13, 2025

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.4K

使用近距离结合试验检测蛋白质-蛋白质相互作用的改进方法.

Zach Tower1, Hao Chang1,2

  • 1Department of Dermatology, University of Wisconsin-Madison, Madison, Wisconsin 53705.

bioRxiv : the preprint server for biology
|September 16, 2024
PubMed
概括

近距离结合试验可以检测蛋白质与蛋白质的相互作用,但可能产生错误的阳性. 本研究确定了这些不准确性,并提供了提高特异性的策略,特别是对于膜蛋白.

科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • 近距离结合试验 (PLA) 是一种敏感的技术,用于检测生物样本中的蛋白质-蛋白质相互作用.
  • 然而,PLA的特异性经常被忽视,导致潜在的不准确性.
  • 假阳性信号可以使蛋白质相互作用研究的解释变得复杂.

研究的目的:

  • 调查和报告近距离结合试验中错误阳性发生的情况.
  • 提供实用建议,以尽量减少PLA中的错误阳性信号.
  • 提高蛋白质-蛋白质相互作用检测的准确性,重点是膜蛋白.

主要方法:

  • 在细胞和组织环境中利用近距离结合试验 (PLA).
  • 系统地分析和量化假阳性信号.
  • 开发和测试方法以减少测试特定的假阳性.

主要成果:

  • 证明近距离结合试验表现出不同程度的虚假阳性结果.
  • 确定了导致这些虚假阳性结果的特定条件和因素.
  • 经过验证的策略有效降低了PLA中错误阳性率.
关键词:
在 Astn2 中使用.在FZD6中使用.蛋白质与蛋白质的相互作用平面细胞的极性是平面的.近距离结合试验测试.

更多相关视频

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
10:05

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

12.8K
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.0K

相关实验视频

Last Updated: Jun 13, 2025

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.4K
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
10:05

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

12.8K
Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

10.0K

结论:

  • 近距离结合试验需要仔细优化以确保特异性.
  • 实施建议的修改可以显著提高蛋白质-蛋白质相互作用检测的准确性.
  • 这些发现对于涉及蛋白相互作用的可靠研究至关重要,特别是对于像膜蛋白这样具有挑战性的目标.