在III型分泌系统表达过程中,Yersinia pseudotuberculosis的生长停止与改变的核糖体蛋白表达和降低的 جنت米辛敏感性有关
Justin Greene1, Rhett A Snyder1, Katherine L Cotten1
1W. Harry Feinstone Department of Molecular Microbiology and Immunology Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.
bioRxiv : the preprint server for biology
|September 16, 2024
概括
耶尔西尼亚型III分泌系统 (T3SS) 导致生长停止,导致核糖体蛋白基因表达减少,并增加了耶尔西尼亚伪结核病的抗生素耐受性.
科学领域:
- 微生物学 微生物学
- 细菌病原体的产生
- 分子生物学分子生物学
背景情况:
- 培养中的Yersinia类型III分泌系统 (T3SS) 的表达与生长停止有关.
- 在Yersinia pseudotuberculosis的指数增长过程中,核糖体蛋白基因非常丰富.
研究的目的:
- 研究T3SS表达对核糖体蛋白基因表达的影响.
- 确定核糖体蛋白基因表达在Yersinia抗生素敏感性中的作用.
主要方法:
- 为核糖体蛋白S10 (rpsJ) 生成一个光转录记者.
- 利用系统性感染和基于滴滴的微流体的小鼠模型.
- 在各种条件下评估细菌生长,T3SS和S10记者表达.
主要成果:
- T3SS表达与感染期间和体外S10水平降低相关.
- 丢失T3SS基因挽救了生长并增加了S10表达.
- T3SS表达赋予了对抗生素 gentamicin 的耐受性.
结论:
- 由T3SS诱导的生长停止减少了Yersinia中的核糖体蛋白基因表达.
- 减少核糖体蛋白表达有助于T3SS活动期间的抗生素耐受性.
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