人类诺罗病毒在健康成年人的宿主内和宿主间的进化
Sasirekha Ramani1, Sara J Javornik Cregeen1,2, Anil Surathu1,2
1Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, 77030, USA.
bioRxiv : the preprint server for biology
|September 16, 2024
概括
在健康的成年人中,人类诺罗病毒的演变显示了个体特异性的变化,而不是广泛的保存突变. 一种新的测序方法成功追踪了病毒基因组,揭示了GI.1菌株的有限免疫压力.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 人类诺罗病毒引起广泛的急性胃肠炎.
- 在免疫功能受损的个体中,诺罗病毒的进化得到了充分的研究.
- 有限的数据存在于健康成年人的诺罗病毒进化动态.
研究的目的:
- 在健康成年人中研究人类诺罗病毒在宿主内部和宿主间的进化.
- 评估一种基于捕获的新型测序方法,用于敏感的诺罗病毒基因组组合.
主要方法:
- 使用了GI.1/Norwalk/US/68病毒对人类感染的受控研究的序列样本.
- 采用特定于诺罗病毒的捕获探针方法,对每个参与者进行最多12个样本的测序.
- 在挑战后长达28天的样本中组装完整的病毒基因组.
主要成果:
- 完整的诺罗病毒基因组甚至从标准检测极限以下的样本中组装起来.
- 在所有参与者中,在GI.1病毒中观察到个体特定的基因组变化.
- 在整个研究队列中没有检测到任何保存的进化变化.
- 在所有病毒蛋白中发生了非同义氨基酸变化,特别是在VP1和NS3.3囊中.
结论:
- 一种敏感的基于捕获的测序方法使得高产率的人类诺罗病毒基因组组装成为可能.
- 在健康的成年人中观察到GI.1诺罗病毒的有限的保存,免疫压力驱动的进化.
- 这些发现有助于理解健康人群中诺罗病毒适应和宿主相互作用.
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