特定染色体的无体积对缺乏螺旋检查点蛋白Bub3的细胞有益
Pallavi Gadgil1, Olivia Ballew2, Timothy J Sullivan3
1Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
bioRxiv : the preprint server for biology
|September 16, 2024
概括
缺乏BUB3基因的细胞可以生存和生长,尽管体积,通常是有害的条件. 这项研究表明,在Bub3枯竭细胞中,特定染色体的增加提供了生存优势.
科学领域:
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 体积,一个异常的染色体数量,通常会损害细胞活力和增殖.
- 然而,特定的基因突变可以导致有益的无体积,挑战这个范式.
- 螺旋组合检查点 (SAC) 确保了精确的染色体分离,防止了形积分.
研究的目的:
- 为了研究形积分症在缺乏螺旋检查点基因BUB3.3的开花酵母细胞中的后果.
- 为了确定Bub3贫乏细胞中的形状是否赋予了生长优势或仅仅是被容忍.
- 鉴定有助于形细胞存活和增殖的基因.
主要方法:
- 生产和培养Bub3缺乏 (bub3Δ) 和Bub1缺乏 (bub1Δ) 酵母菌株.
- 通过使用遗传和显微镜技术,分析多代染色体数量和稳定性.
- 评估基因表达特征和体细胞与体细胞的生长率.
主要成果:
- 在长时间内,Bub3缺乏细胞对特定染色体表现出稳定的无体积变异.
- 与野生类型细胞相比,BUB3和BUB1的损失增加了染色体误分率.
- 参与染色体分离和细胞周期调节的某些基因的高表达赋予了Bub3贫乏细胞的选择性优势.
结论:
- 在Bub3贫乏酵母中,积体并不有害,并且可以在几代人中保持.
- 特定动脉质病的持久性表明一种有益的作用,可能是通过基因剂量效应.
- 通过积分重点基因的升级可以促进染色体分离忠实度受损的细胞的存活和生长.
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