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Updated: Jun 13, 2025

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
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CryptKeeper:一种负面设计工具,用于减少细菌中的无意基因表达

Cameron T Roots1, Jeffrey E Barrick1

  • 1Department of Molecular Biosciences, Center for Systems and Synthetic Biology, The University of Texas at Austin, Austin, Texas 78712, U.S.A.

bioRxiv : the preprint server for biology
|September 16, 2024
PubMed
概括

CryptKeeper软件预测合成DNA的意外基因表达,防止基因不稳定性和分子生物学中的实验失败. 这种工具有助于研究人员设计实验,避免神秘基因表达的并发症.

关键词:
计算的DNA序列设计设计.设计 - 建造 - 测试周期.质粒的不稳定性 质粒的不稳定性再组合蛋白过度表达的情况.可靠性和可重复性.

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