Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Side-to-side functional symmetry and radiographic stability after distal ulna resection with ECU tenodesis: A case series of grade III giant cell tumors.

Journal of clinical orthopaedics and trauma·2026
Same author

Neoadjuvant docetaxel, cisplatin, and capecitabine in borderline-inoperable locally advanced oral cavity squamous cell carcinoma: A prospective observational study.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2026
Same author

The SMARCA4 subunit of the SWI/SNF complex prevents genome instability at G quadruplexes.

Genome biology·2026
Same author

Tumour specific HORMAD1 expression perturbs mitotic arrest and drives sensitivity to mitotic kinase inhibitors.

Nature communications·2026
Same author

EZH2 inhibition overcomes immunomodulatory drug resistance in multiple myeloma via a cereblon-dependent pathway.

Haematologica·2026
Same author

Neuroblastoma: Anti-Invasive Effects of Tetracaine In Vitro.

Basic & clinical pharmacology & toxicology·2026
Same journal

Elevated urea levels in human frontotemporal dementia and amyotrophic lateral sclerosis post-mortem brain tissue: Evidence of a multi-dementia pathogenic mechanism.

Molecular omics·2026
Same journal

Metabolite-centric identification of antimetabolite drug targets across cancer and neurodegenerative diseases.

Molecular omics·2026
Same journal

Platelet proteomics on less than a drop of previously frozen, non-citrate plasma.

Molecular omics·2026
Same journal

Decoding the diabetic transition: a lipidomic approach for biomarker discovery in an Indian cohort.

Molecular omics·2026
Same journal

Placental metabolomics for assessment of pregnancy complications: a systematic review.

Molecular omics·2026
Same journal

Prostate cancer: metabolic remodelling in expressed prostatic secretions reveals cellular structural changes measured by mpMRI.

Molecular omics·2026
查看所有相关文章

相关实验视频

Updated: Jun 13, 2025

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
08:06

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Published on: November 10, 2016

7.6K

YAP激活对稀释具有强大的强度.

Ian Jones1, Mar Arias-Garcia1, Patricia Pascual-Vargas1

  • 1Chester Beatty Laboratories, Division of Cancer Biology, Institute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK. dmcbijo@ucl.ac.uk.

Molecular omics
|September 16, 2024
PubMed
概括
此摘要是机器生成的。

细胞大小的变化不会影响YAP蛋白质.

更多相关视频

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
11:30

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Published on: January 20, 2017

11.5K
Rapid Characterization of Genetic Parts with Cell-Free Systems
05:00

Rapid Characterization of Genetic Parts with Cell-Free Systems

Published on: August 30, 2021

1.8K

相关实验视频

Last Updated: Jun 13, 2025

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
08:06

Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays

Published on: November 10, 2016

7.6K
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
11:30

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Published on: January 20, 2017

11.5K
Rapid Characterization of Genetic Parts with Cell-Free Systems
05:00

Rapid Characterization of Genetic Parts with Cell-Free Systems

Published on: August 30, 2021

1.8K

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 癌症研究 癌症研究

背景情况:

  • 由于细胞的合成,降解和细胞大小,转录因子度在细胞之间有显著的变化.
  • 转录因子功能对度波动的稳定性以及背后的机制尚不清楚.

研究的目的:

  • 研究细胞大小如何影响YAP转录因子的度和核转位.
  • 探索调节YAP度和跨不同细胞类型和条件的转移的机制.

主要方法:

  • 在乳腺癌细胞中使用成像技术量化全细胞和核YAP度.
  • 分析了与细胞面积,DNA含量和细胞周期标记物 (CycA/PCNA) 相关的YAP度.
  • 综合成像与基蛋白质数据,并使用化学抑制剂 (MEK,CDK4/6) 来研究YAP调节.

主要成果:

  • 随着细胞面积的增加,全细胞YAP度下降,但核YAP度在细胞大小中保持不变.
  • YAP度与DNA含量和细胞周期标志物相关,表明细胞周期合成抵消稀释.
  • RAS/MAPK信号,焦粘附成熟和核运输预测了核YAP水平;MEK和CDK4/6抑制增加了核YAP.

结论:

  • YAP核转位对由细胞大小变化引起的全细胞度变化具有强度.
  • 细胞循环依赖的合成和信号通路 (RAS/MAPK) 调节YAP核进口,尽管细胞生长,但仍保持功能.
  • 这项研究表明,尽管细胞质稀释,蛋白质转移到细胞核的机制仍然存在.