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主要肝细胞的隔离和冷保存

Xiaoqing Guo¹, Ji-Eun Seo¹, Kelly Davis²

¹Division of Genetic and Molecular Toxicology, National Center for Toxicological Research, Jefferson, Arkansas.

Current protocols

|September 16, 2024

概括

主要肝细胞 (PMHs) 为药物毒性测试提供了人类细胞的可行替代方案. 这种方法详细介绍了它们的隔离,冷保存和用于临床前研究的恢复.

科学领域:

肝病学肝病学是一种肝病学.
毒理学毒理学毒理学
灵长类动物研究研究

背景情况:

初级人类肝细胞 (PHHs) 是体外药物毒性评估的黄金标准.
由于PHHs的有限可用性,需要替代模型.
与人类相比,动物肝细胞显示出显著的药物代谢差异,与非人类灵长类动物不同.

研究的目的:

描述一种从 rhesus (Macaca mulatta) 中分离初级肝细胞的方法.
详细介绍冷保存和恢复这些原始肝细胞 (PMHs) 的技术.
在毒理学和临床前研究中,确定PMH作为PHH的可靠替代品.

主要方法:

在位整体肝脏 perfusion 的 rhesus 的肝脏.
隔离和细胞涂层的初级肝细胞.
对于PMHs的冷保存和恢复协议.

主要成果:

成功隔离了可行的原始肝细胞.
有效的冷保存和恢复PMH功能.
证明了和人类之间的生理和遗传相似性.

结论:

描述的协议提供了一种可靠的方法来获得PMHs.
在药物毒性和临床前研究中,PMHs可以作为PHHs的宝贵替代品.
这一进步支持更准确的体外毒理学评估.

关键词:

冷保存的方法肝细胞隔离肝细胞隔离肝脏 perfusion perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 肝脏 perfusion 主要的肝细胞.

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