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拜卡莱因通过向Candida auris中的核糖体来诱导亡

Can Li^1,2, Jun Wang³, Hui Wu^1,2

¹Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, (College of Life Science), Anhui University of Chinese Medicine, No. 350 Longzihu Road, Xinzhan District, Hefei, 230012, China.

Archives of microbiology

|September 16, 2024

概括

拜卡莱因 (BE) 通过触发亡并破坏线粒体功能,有效地对抗危险的"超级真菌"Candida auris. 这种天然化合物显示出作为一种新型抗真菌治疗的希望,甚至针对耐药菌株.

科学领域:

菌类学菌类学是指菌类学.
药理学药理学是指药理学的学科.
分子生物学分子生物学

背景情况:

黄是一种多药耐药的真菌病原体,会引起严重的感染.
现有的抗真菌治疗方法有限,需要新的治疗策略.
拜卡莱因 (BE) 是从Scutellaria baicalensis中提取的,它对C. auris.有抗真菌的功效.

研究的目的:

为了研究 Baicalein (BE) 对Candida auris.的抗真菌活性.
阐明BE在C. auris.中的作用机制.
探索BE作为C. auris感染的潜在治疗剂.

主要方法:

扫描电子显微镜 (SEM) 检测形态变化.
亡检测试验 (附录V-FITC,CaspACE FITC-VAD-FMK,TUNEL) 进行检测.
测量反应性氧物种 (ROS),单片氧和线粒体膜潜力 (MMP).
转录组分析和RT-qPCR用于基因表达造型.

主要成果:

在C. auris中,BE诱导了显著的亡特征,包括酸胺外化,元酶激活,核凝结和DNA碎片化.
BE治疗导致细胞内ROS水平增加,并改变了线粒体功能.
转录组分析表明,BE影响与核糖体相关的途径,可能将核糖体识别为新型抗真菌标.

结论:

拜卡莱因通过诱导细胞亡和破坏线粒体功能,表现出强烈的抗真菌活性,可以对抗Candida auris.
这些发现表明,与核糖体相关的途径参与BE诱导的C. auris亡.
巴伊卡莱因代表了开发新抗真菌疗法的有希望的候选者,用于对抗C. auris感染.

关键词:

它们是Candida auris.细胞灭亡 (apoptosis) 是一种死亡的过程.在 Baicalein 的酒吧里核糖体核糖体核糖体核糖体文字转录学 (Transcriptomics) 是一个学科.

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