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相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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RNA-seq03:21

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: Jun 13, 2025

Robust 3D DNA FISH Using Directly Labeled Probes
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对DNA/RNA/免疫光 (IF) -seqFISH数据的图像分析协议

Hiroaki Ohishi1, Hiroshi Ochiai2

  • 1Division of Gene Expression Dynamics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.

Methods in molecular biology (Clifton, N.J.)
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概括

这项研究详细介绍了基于成像的空间多组学,重点是序列光 in situ 杂交 (seqFISH). 它提供了一种实用的图像分析协议,用于观察细胞和组织内的基因转录和蛋白质定位.

关键词:
高阶基因组结构的更高阶基因组结构.图像成像技术可以提供图像.转录 翻译 翻译这就是为什么seqFISH.

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Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
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相关实验视频

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 细胞生物学 细胞生物学

背景情况:

  • 基于成像的空间多基因组学能够分析基因组结构,基因转录和蛋白质定位在单个基因基因水平上.
  • 这些技术提供了对生物现象的详细观察,包括细胞内转录机制.

研究的目的:

  • 详细介绍基于成像的空间多omics技术的原理.
  • 为顺序光 in situ杂交 (seqFISH) 图像分析提供一个全面的协议.
  • 通过可访问的代码实现实际应用.

主要方法:

  • 专注于DNA / RNA / 免疫光 (IF) 序列光在位杂交 (seqFISH).
  • 图像分析的详细一步一步协议:预处理,点检测和数据可视化.
  • 为实际应用提供完整的Jupyter Notebook代码.

主要成果:

  • 展示空间多主题分析的原则.
  • 这里介绍了一个用于seqFISH图像分析的功能协议.
  • 开放获取代码可以促进可重复的研究.

结论:

  • 基于成像的空间多组学,特别是seqFISH,是生物研究的强大工具.
  • 提供的协议和代码提高了这些先进技术的可访问性和应用.
  • 便于在细胞和组织环境中详细观察分子事件.