通过电膜提取与液态色谱相结合的尿液中氧多环芳的确定
Haidong Gao1, Shucai Gao1, Ming Lv1
1Institute of Business Scientific, Henan Academy of Sciences, Wenhua Road #87, Zhengzhou, Henan 450003, China.
Journal of chromatography. A
|September 16, 2024
概括
使用电膜提取 (EME) 的新方法准确地测量尿液中的氧多环芳 (OH-PAHs). 这种技术可以提高这些致癌生物标志物的检测,无论是吸烟者还是非吸烟者.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 生物标记分析 生物标记分析
背景情况:
- 多环芳 (PAH) 是已知的环境致癌物.
- 尿液中的氧多环芳 (OH-PAH) 作为PAH暴露的关键生物标志物.
- 需要准确和敏感的方法来确定尿路OH-PAH.
研究的目的:
- 开发一种简单,有效和环保的尿液OH-PAHs样品预处理技术.
- 建立一种敏感的分析方法,用于量化人类尿液中的四种典型OH-PAHs.
- 评估该方法在生物样本中分析OH-PAH的性能.
主要方法:
- 电膜提取 (EME) 用于选择性提取,净化和丰富OH-PAHs.
- 为了分析,EME与高性能液体染色学 (HPLC) 相结合.
- 优化了EME条件,以实现高灵敏度和恢复.
主要成果:
- 开发的EME-HPLC方法证明了四种OH-PAH的宽线性范围 (1-500 ng mL-1).
- 在0.05-0.3 ng mL−1之间实现了低检测极限 (LODs).
- 获得了高平均恢复率 (81.6-102.5%) 与较低的相对标准偏差 (RSDs <9.4%).
结论:
- EME-HPLC方法是一种灵敏而准确的技术,用于确定人类尿液中的微量OH-PAHs.
- 该方法已成功应用于分析吸烟者和非吸烟者的尿样.
- 这种方法显示出在各种生物样本中分析OH-PAHs的巨大潜力.
更多相关视频
07:26A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
Published on: January 1, 2016
13.7K
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
584
相关概念视频
01:05High-Performance Liquid Chromatography: Elution Process
436
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
436
01:15High-Performance Liquid Chromatography: Types of Detectors
507
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
507
01:19Gas Chromatography: Types of Detectors-II
344
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
344
01:11High-Performance Liquid Chromatography: Introduction
1.6K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
1.6K
00:56Extraction: Advanced Methods
432
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
432
01:30Capillary Electrophoresis: Applications
350
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
350