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血清衍生的蛋白质冠状体会影响纳米粒子与大脑细胞的相互作用
Nabila Morshed1, Claire Rennie1, Wei Deng2
1School of Life Sciences, University of Technology Sydney, Sydney, NSW 2007, Australia.
Nanotechnology
|September 16, 2024
概括
蛋白质冠状体显著影响纳米颗粒与大脑细胞的相互作用. 了解这些蛋白质涂层对于推进神经神经医学和开发有效的纳米疗法至关重要.
科学领域:
- 神经医学是神经医学.
- 纳米粒子-细胞相互作用
- 生物材料科学是生物材料的科学.
背景情况:
- 神经医学组合了神经科学和纳米疗法用于大脑治疗.
- 由于对蛋白质冠状病毒效应的理解有限,神经神经医学的临床翻译受到阻碍.
- 蛋白质冠状体影响纳米粒子的行为和生物系统内的相互作用.
研究的目的:
- 研究血清衍生蛋白质冠状体对聚合物纳米粒子与脑衍生细胞相互作用的影响.
- 为了表征聚氨酸-乳糖-糖酸 (PLGA) 和PLGA-聚乙烯糖醇 (PLGA-PEG) 纳米颗粒上的蛋白质冠状组成.
- 评估冠状涂层纳米颗粒对微质,神经元细胞和星体细胞的影响.
主要方法:
- 在PLGA和PLGA-PEG纳米粒子上形成血清衍生蛋白质冠状体.
- 使用液体染色学-质谱学对蛋白质冠状体的表征.
- 在初级脑细胞培养物中评估纳米粒子内部化和细胞反应 (细胞因子释放,活力).
主要成果:
- 对于PLGA (高环球蛋白,阿波利波蛋白) 和PLGA-PEG (高蛋白酶抑制剂) 纳米颗粒,确定了不同的蛋白冠状组合.
- 冠状病毒涂层的PLGA纳米粒子被微质细胞和神经元细胞内化,但不是天体细胞.
- 纳米颗粒内部化诱导了促炎性细胞因子的释放,并降低了神经元的活力,令人惊的是,冠状涂层纳米颗粒被拯救了.
结论:
- 蛋白质冠状体在调解纳米粒子与特定类型的大脑细胞相互作用方面发挥着关键作用.
- 了解蛋白质冠状元的组成对于设计有效的神经元医药至关重要.
- 对冠状病毒中介效应的进一步研究可以指导针对神经疾病的向纳米疗法的开发.
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