相关实验视频

Updated: Jun 13, 2025

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells

Published on: June 5, 2015

模块化半合成方法从重组IgG1抗体生成T细胞依赖的双特异构造

Irene Shajan¹, Léa N C Rochet², Shannon R Tracey³

¹Laboratory of Organic Chemistry, Wageningen University & Research, Stippeneng 4, Wageningen 6807 WE, The Netherlands.

Bioconjugate chemistry

|September 16, 2024

概括

我们开发了一种用于癌症免疫治疗的新型双特异抗体平台. 这个平台产生强大的T细胞依赖双特异性抗体 (TDBAs),向HER2瘤和CD3T细胞,提供增强的瘤细胞杀伤,并减少副作用.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物化学生物化学

背景情况:

双特异性抗体将T细胞重定向到瘤细胞,用于癌症治疗.
依赖T细胞的双特异性抗体 (TDBAs) 是一种强大的免疫治疗药物.
现有的TDBAs可以导致细胞因子释放综合征由于CD3受体结合.

研究的目的:

开发一种用于产生2:1T细胞依赖双特异抗体 (TDBAs) 的新平台.
创建具有对HER2受体双价值和对CD3受体单价值的TDBA.
为了提高瘤杀伤效率,同时最大限度地减少诸如细胞因子释放综合征等副作用.

主要方法:

利用生物的逆电子需求迪尔斯-阿尔德 (IEDDA) 点击反应对遗传编码的氨酸残留物.
在批准的抗体trastuzumab上安装了一个TCO手柄.
进行TCO-四素点击化学与CD3结合的Fab,以创建2:1的HER2 × CD3TDBA.

主要成果:

成功生成了一个新的 2:1 HER2 × CD3 TDBA 构造.
在Picomolar度下,TDBA表现出强大的瘤杀伤能力.
建议使用单价CD3结合来减少细胞因子释放综合征.

结论:

一个多功能半合成平台能够快速生成强大的TDBA构造.
开发的2:1TDBA在向HER2表达瘤细胞方面表现出高效.
这种方法为开发更安全,更有效的癌症免疫疗法提供了一个有希望的策略.

更多相关视频

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Generation of Murine Monoclonal Antibodies by Hybridoma Technology

Published on: January 2, 2017

相关概念视频

Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...

Diversity of Antigen Receptors

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...