路西法酶报告员测试NF-kB激活由开源流体处理平台自动化
1Opentrons Labworks Inc., New York, NY 11101, USA.
SLAS technology
|September 16, 2024
概括
我们自动化了一种 luciferase 记者测定,用于对基因调节的高通量分析. 这种方法有效地通过机器人自动化测量细胞中核因子kappa B (NF-kB) 的激活.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 酶学 是一种酶学.
背景情况:
- 路西法酶记者测定是基于细胞的酶性实验,测量转录活性.
- 这些测试利用 luciferase 和 luciferin 产生发光,表明基因调节.
- 它们可以适应高通量选,并且广泛用于研究.
研究的目的:
- 为了自动化 luciferase 记者测试以测量 NF-kB 激活.
- 在Opentrons OT-2平台上开发基于Python脚本的机器人处理协议.
- 为了提高效率并减少记者基因测定中的实践时间.
主要方法:
- 使用Opentrons的OT-2液体处理平台,自动化进行化酶记者测试.
- 开发用于细胞播种,转染和酶反应的Python脚本.
- 测量发光度以量化HeLa细胞中NF-kB通路激活.
主要成果:
- 成功自动化了 luciferase 记者测试工作流程.
- 在HeLa细胞中证明了NF-kB激活的准确测量.
- 通过最小的手动干预,实现了高质量的样品处理.
结论:
- 机器人自动化 luciferase 记者测定是可行的和高效的.
- 开发的协议使得高通量,可重复的基因激活测量.
- 这种自动化系统减少了实践时间,并改善了记者测试的样本处理.
更多相关视频
10:13A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
Published on: July 3, 2013
11.1K
10:57NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
Published on: January 12, 2020
10.5K
相关概念视频
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
02:26NF-kB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...