通过数千个mRNA未翻译区域的转录后重编程,在三子体中进行了重编程
Anna Trenaman1, Michele Tinti1, Richard J Wall1,2
1The Wellcome Centre for Anti-Infectives Research, School of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.
Nature communications
|September 16, 2024
概括
研究人员在非洲试管体中发现了数千个调节信使RNA未翻译区域 (UTR). 这些UTRs,特别是聚纯素通道,控制了这种寄生虫的基因表达,揭示了一个新的cis-regulatory代码.
科学领域:
- 分子生物学分子生物学
- 寄生虫学的寄生虫学
- 遗传学 遗传学 是一个
背景情况:
- 试类体表现出全基因组的多基因组转录,强调转录后的基因调节.
- 这些生物中的信使RNA (mRNA) cis-调节性未翻译区域 (UTR) 仍然在很大程度上没有表征.
- 了解UTR功能对于破译三子体中基因表达控制至关重要.
研究的目的:
- 在非洲试管体中以全基因组规模识别和表征调控性UTR.
- 研究特定序列元素,如多纯素通道 (pPuTs) 在UTR介导的基因调节中的作用.
- 为了揭示控制三子体基因表达的cis-regulatory序列代码.
主要方法:
- 用基因组规模的大规模并行记者分析 (MPRA) 来分析3'-UTRs.
- 3'-UTR-seq分析与MPRA结合,确定了数千个监管性UTR.
- 机器学习预测用于对原生和合成UTR进行独立评估.
主要成果:
- 数以千计的调节性3'-UTRs在非洲试管体中被发现.
- 翻译效率的提高与富含腺因的多纯素通道 (pPuTs) 的存在相关.
- 3'-UTRs中的富含乌拉的聚胺系与血流阶段细胞的上调表达有关,这表明发育调节.
结论:
- 一个cis-regulatory UTR序列代码存在于三子体中,控制构成性转录基因组内的基因表达.
- 成千上万的UTRs的功能是通过后转录重新编程三子体中的基因表达特征.
- 聚氨酸和聚胺片段代表了本UTR介导的监管代码中的关键元素.
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