相关实验视频

Updated: Jun 13, 2025

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling

Published on: May 31, 2017

CCL5对于轴突发生和脑损伤后的神经元恢复至关重要

Man-Hau Ho^1,2, Yih-Jeng Tsai^3,4, Chia-Yen Chen²

¹Program in Medical Neuroscience, College of Medical Science and Technology, Taipei Medical University and National Health Research Institute, Taipei, 11031, Taiwan.

Journal of biomedical science

|September 16, 2024

概括

在创伤性脑损伤 (TBI) 后,CCL5促进神经元的修复,通过增强轴突和突触再生. 这项研究突出了CCL5的特点.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
再生医学是一种再生医学.

背景情况:

创伤性脑损伤 (TBI) 导致轴突和突触损伤,导致神经系统缺陷和神经退行.
轴突和突触修复对于创伤后的功能恢复至关重要.
C-C 基因化学基因连接物5 (CCL5) 具有神经保护性.

研究的目的:

研究CCL5在TBI后神经元再生中的作用.
评估CCL5在治疗TBI诱导的轴突损伤和退化中的治疗潜力.

主要方法:

在野生型和CCL5淘汰赛小鼠中使用重量下降模型诱导轻度TBI.
通过行为测试 (mNSS,旋转杆,梁行走,贴纸删除) 评估神经功能.
通过戈尔吉染色和对突触蛋白质的评估 (synaptophysin,PSD95) 分析了轴突和突触完整性.
重组CCL5被注射入鼻,作为治疗前或后的治疗.
蛋白质组分析 (LC-MS/MS),免疫染色和西部斑被用来识别和量化信号通路.
主要神经元培养物被用来研究生长的形成和信号传递.

主要成果:

经过CCL5淘汰赛的小鼠在TBI后表现出运动和感觉功能的恢复受损,轴突胀和突触蛋白减少.
在CCL5-KO TBI小鼠中,鼻内CCL5的使用改善了神经功能,并恢复了突触完整性.
蛋白质组分析显示,CCL5-KO小鼠的"神经系统发育和功能"途径减少,CCL5治疗增强了这些途径.
CCL5上调调节了轴突发生,突触发生和髓化信号通路,包括Semaforin,Ephrin,mTOR,Neuregulin/ErbB和FGF/FAK.
通过CCR5,mTOR和Rho信号传递,CCL5促进了生长状类动物的形成.
长期使用CCL5导致皮质重建,并增加了Reelin和CXCR4的表达.

结论:

在TBI后,CCL5对于启动内在神经元再生系统至关重要,包括生长形,轴突发生,突触发生和复髓化.
在受伤后,CCL5促进皮质电路的适当重新连接.
CCL5代表了一种有前途的治疗策略,用于治疗轻度TBI后的慢性阶段的轴突损伤和退化.

关键词:

艾克森的伤害是因为Axon的伤害.轴突生成 (Axonogenesis) 是一种作用于细胞的过程.CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 骨髓化骨髓化创伤性脑损伤是一种创伤性脑损伤.

更多相关视频

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Published on: March 30, 2018

相关概念视频

Neurogenesis and Regeneration of Nervous Tissue

Neurogenesis and Regeneration of Nervous Tissue

In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...