冷保存的人类精子的储存时间不会影响参与生育的途径
Sara Stigliani1, Adriana Amaro2, Francesco Reggiani2
1SS Physiopathology of Human Reproduction, IRCCS Ospedale Policlinico San Martino, Genova, Italy.
Basic and clinical andrology
|September 16, 2024
概括
为了保护生育能力,冷保存人类精子不会影响生育途径,无论储存时间如何. 与短期存储 (1周) 相比,长期冷存储 (中位数为9年) 显示出最小的转录组变化.
科学领域:
- 生殖生物学 生殖生物学
- 精子冷生物学的精子
- 分子遗传学 分子遗传学
背景情况:
- 人类精子冷保存对于辅助生殖,生育保护和精子捐赠至关重要.
- 冷保存可以诱导精子损伤,包括DNA损伤和转录组改变.
- 了解储存时间对精子质量的影响至关重要.
研究的目的:
- 为了评估冷存储时间对人类精子转录组的影响.
- 为了确定长时间冷保存精子是否会影响与生育相关的途径.
主要方法:
- 来自24名精子捐赠者的精液样本被冷保存了短期 (1周) 和长期 (中位数为9年).
- 从冷解的精子中提取RNA,并使用微阵列进行聚合和分析.
- 进行了功能注释丰富分析.
主要成果:
- 与短期存储组相比,长期存储组的五个转录显示出更高的丰度.
- 没有观察到冷保存时间对调节精子生理和功能的关键通路产生显著影响.
- 功能性注释显示,对与生育相关的途径没有影响.
结论:
- 对于人类精子来说,冷保存的持续时间不会对生育必不可少的途径产生不利影响.
- 长期冷储存是精子储存的可行选择,而不会影响途径层面的生育潜力.
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