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相关概念视频

RNA Splicing01:32

RNA Splicing

56.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.2K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.2K
5.2K
Alternative RNA Splicing02:18

Alternative RNA Splicing

21.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.0K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

2.7K
2.7K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.2K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
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相关实验视频

Updated: Jun 13, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

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斯普拉姆:一种基于深度学习的拼接位置预测器,可以改善拼接对齐.

Kuan-Hao Chao1,2, Alan Mao3,4,5, Steven L Salzberg3,4,5,6

  • 1Department of Computer Science, Johns Hopkins University, Baltimore, MD, 21218, USA. kh.chao@cs.jhu.edu.

Genome biology
|September 16, 2024
PubMed
概括

我们开发了Splam,这是一种新的深度学习方法,用于预测DNA中的拼接接口. 在人类拼接接口预测中,Splam 实现了96%的准确性,超过了现有的方法.

关键词:
卷积神经网络是一种卷积神经网络.深度学习是一种深度学习.扩展的卷积卷积.剩余的区块是剩余的拼接位置预测器拼接的对齐方式是拼接对齐.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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相关实验视频

Last Updated: Jun 13, 2025

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • 使者RNA (mRNA) 拼接是基因表达的关键过程,涉及去除内子以形成成熟的mRNA.
  • 结结,即外子和内子之间的边界,对于精确的基因和基因变异形成至关重要.
  • 准确预测结结点对于理解基因调节和疾病机制至关重要.

研究的目的:

  • 介绍Splam,一种新的深度学习方法,用于预测DNA中的拼接接口.
  • 提高拼接连接预测模型的准确性和生物相关性.
  • 为分析基因拼接过程提供更有效的工具.

主要方法:

  • 开发Splam,一个深度残余卷积神经网络模型.
  • 采用400个基对的窗口,在每个接部位旁边,以捕获相关的生物信号.
  • 同时在供体和接受体拼接位上同时训练模型,以模仿拼接机器的识别过程.

主要成果:

  • 斯普拉姆在预测人类拼接连接方面表现出很高的准确性,达到96%的准确性.
  • 该方法在预测准确性方面优于现有模型,例如SpliceAI.
  • 该模型的设计,包括横向的序列窗口和联合培训,反映了生物拼接的关键方面.

结论:

  • 斯普拉姆代表了拼接接头预测准确性的重大进步.
  • 该方法的生物知情方法提高了其在基因组研究中的可靠性和实用性.
  • 斯普拉姆为研究基因拼接及其在遗传多样性和疾病中的作用提供了一个强大的新工具.