相关实验视频

Updated: Jun 13, 2025

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets

Published on: May 15, 2017

探索pH触发的状到立方相转换在2-氧烯酸/单烯酸纳米分散:洞察膜物理化学性质

Xuehui Rui¹, Nozomi Morishita Watanabe¹, Yukihiro Okamoto¹

¹Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyamacho, Toyonaka, Osaka 560-8531, Japan.

The journal of physical chemistry. B

|September 17, 2024

概括

这项研究揭示了脂质膜性质如何在脂质纳米粒子 (LNP) 的pH触发过渡过程中发生变化. 了解这些变化是开发先进的pH响应药物递送系统的关键.

科学领域:

纳米技术和材料科学材料科学
生物物理学的生物物理.
药物输送系统药物输送系统

背景情况:

自组装的脂质纳米粒子 (LNP) 是药物输送的关键纳米载体.
使用可离子化脂质的功能化产生了对pH响应的LNP,用于触发药物释放.
现有的研究往往忽略了在LNP阶段过渡期间的脂质膜特性.

研究的目的:

为了研究pH诱导的状到立方相过渡期间脂质膜的物理化学性质变化.
探索LNP相结构和脂质膜行为之间的关系.
为设计改进的pH响应药物递送平台提供见解.

主要方法:

使用2-氧烯酸/单烯酸 (2-OHOA/MO) 制备pH响应的LNP.
微角X射线散射 (SAXS) 来确定相位过渡 (从状到立方).
劳尔丹和DPH光探针来评估脂质-水界面极性和膜流动性.
拉曼光谱分析脂质链包装和扭曲.

主要成果:

萨克斯证实了依赖于pH的从叶片囊泡 (Lα) 到立方体 (Im3m/Pn3m) 的过渡.
光探针显示了与相位过渡相关的界面极性和膜流动性的变化.
拉曼光谱检测显示过渡期间脂质链包装和扭曲的变化.
在2-OHOA/MO系统中,观察到的脂质膜特性变化与状到立方相过渡相吻合.

结论:

在2-OHOA/MO LNP中,在pH触发的叶片到立方相过渡过程中,脂膜物理化学特性显著变化.
阶段结构和膜特性之间的相互作用对LNP行为至关重要.
这些发现为开发更有效,更可靠的pH响应药物递送系统提供了宝贵的指导.

更多相关视频

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes

Published on: February 19, 2016

相关概念视频

Membrane Fluidity

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.

Colloids

Colloids

Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...

Fluid Mosaic Model

Fluid Mosaic Model

Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...

Phase Transitions: Vaporization and Condensation

Phase Transitions: Vaporization and Condensation

The physical form of a substance changes on changing its temperature. For example, raising the temperature of a liquid causes the liquid to vaporize (convert into vapor). The process is called vaporization—a surface phenomenon. Vaporization occurs when the thermal motion of the molecules overcome the intermolecular forces, and the molecules (at the surface) escape into the gaseous state. When a liquid vaporizes in a closed container, gas molecules cannot escape. As these gas phase...