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Updated: Jun 13, 2025

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基于LC-MS的策略,用于化学分析和量化Ginkgo biloba种子的配送颗粒

Facheng Zhang¹, Qingqing Fei^2,3, Xiaojun Huang^2,3

¹Polifarma (Nanjing) Co., Ltd., Nanjing, 210038, PR China.

|September 17, 2024

概括

这项研究确定了Ginkgo biloba种子分发颗粒 (GSDG) 中的56种化学化合物,这是中国传统医学的. 它建立了一种定量方法来分析关键的生物活性植物化学物质,确保对这种新型草药的质量控制.

科学领域:

植物化学植物化学
分析化学分析化学
传统中国医药传统中国医药

背景情况:

银杏花种子是一种传统的中医药,用于治疗呼吸道和妇科疾病.
银杏种子分发颗粒 (GSDG) 是一种在诊所广泛使用的新型配方.
GSDG的化学成分尚未完全描述,这阻碍了进一步的研究.

研究的目的:

为了全面描述GSDG的化学特征.
开发和验证一种定量方法,用于在GSDG.中确定生物活性植物化学物质.
为GSDG准备,质量控制和理解其作用机制提供基础.

主要方法:

高性能液体色谱与四极飞行时间质谱学 (HPLC-QTOF-MS) 结合用于组件识别.
高性能液体色谱与多重反应监测 (MRM) 模式的四极线性离子陷质谱学 (HPLC-QIT-MS) 结合,用于定量分析.
根据既定指南验证方法.

主要成果:

总共有56种化合物被确定在GSDG中,包括氨基酸,有机酸,核化物,黄类,维生素,类乳和碳水化合物.
选择了用于质量控制的关键分析物,包括谷氨酸,酸,histidine,ginkgolides A,B,C和J,eucomic acid,以及两个新的N-glycosyl indoleacetyl化合物.
$N$-(N-glucopyranosyl) -indoleacetylaspartate是最丰富的植物化学物质 (17.325.7 mg/g),而金戈利德J是最不丰富的 (0.01970.0335 mg/g).

结论:

该研究提供了GSDG的全面化学概况.
一种经过验证的定量方法使GSDG的可靠质量控制成为可能.
这些发现支持标准化和进一步研究GSDG的治疗潜力.

关键词:

用于分配金科比洛巴种子的颗粒.这就是LC-MS.定性分析是一种定性分析.定量分析是一种量化分析.

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