相关实验视频
Updated: Jul 4, 2026
09:07
An Orthotopic Model of Murine Bladder Cancer
Published on: February 6, 2011
15.9K
在人性化的肝脏小鼠中,4,4'-甲基2-氨) 的尿囊致癌潜力
Shugo Suzuki1, Min Gi1,2, Takuma Kobayashi3
1Department of Molecular Pathology, Osaka Metropolitan University Graduate School of Medicine, Osaka 545-8585, Japan.
Toxicological sciences : an official journal of the Society of Toxicology
|September 17, 2024
概括
职业接触4,4'-甲基二2-乙烯 (MOCA) 会增加膀癌的风险. 人性化肝脏小鼠显示MOCA诱导的泌尿器损伤和更高的N-OH-MOCA水平,表明其在人类致癌过程中的作用.
科学领域:
- 毒理学 毒理学 毒理学
- 致癌的发生是致癌的产生.
- 代谢过程中的代谢.
背景情况:
- 职业暴露于4,4'-甲基2-氨 (MOCA) 与增加尿膀癌风险有关.
- 了解MOCA的代谢途径对于评估其致癌潜力至关重要.
研究的目的:
- 用人性化肝脏小鼠模型研究MOCA代谢在膀癌发生中的作用.
- 为了比较MOCA诱导的尿囊表皮和代谢物形成在人性化的肝脏与非人性化的小鼠.
主要方法:
- 给非人性化 (F1-TKm30) 和人性化肝脏小鼠4周和28周的MOCA.
- 对MOCA诱导的代谢酶表达,代谢物形成和尿路效应的变化进行比较.
- 分析尿中的代谢物度,特别是N-OH-MOCA.
主要成果:
- 人性化肝脏小鼠在MOCA暴露4周后表现出尿路细胞增生,增殖和DNA损伤,与非人性化小鼠不同.
- 在4周后,人性化肝脏小鼠的尿液N-OH-MOCA度比非人性化小鼠高10倍以上.
- 在两个模型之间观察到细胞染色体P450异型表达的明显差异.
结论:
- 建议N-OH-MOCA是MOCA在人类中的致癌潜力的重要贡献者.
- 人性化肝脏小鼠模型有效地模仿了MOCA诱导的早期膀组织变化,与人类暴露相关.
更多相关视频
相关概念视频
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...