依赖的酸盐输送体PiT1/SLC20A1作为内源逆转录病毒包裹syncytin-B的受体,参与小鼠胎盘形成
Guillaume Mousseau1, Noémie Préault2, Sylvie Souquere3
1Viroxis, Institut Gustave Roussy, Villejuif, France.
Journal of virology
|September 17, 2024
概括
研究人员确定PiT1/SLC20A1是小鼠syncytin-B (SynB) 的细胞受体,这是一种对胎盘发育至关重要的蛋白质. 这一发现解释了SynB的存在.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 合成素是对胎盘发育至关重要的逆转录病毒包膜基因.
- 鼠标的植入过程涉及两个同聚类细胞层 (ST-I和ST-II),每个层都表达不同的同聚素:SynA和SynB.
- 已知SynA的受体,但SynB受体仍未确定.
研究的目的:
- 为了识别小鼠syncytin-B (SynB) 的细胞受体.
- 阐明已识别的受体在SynB介导的细胞融合和胎盘形成中的作用.
主要方法:
- 细胞融合试验结合了对人类ORFeome衍生表达库的选.
- 用克隆受体基因感染细胞.
- 免疫沉以确认蛋白质相互作用.
- PiT1/PiT2 嵌合体和截断实验.
- 对PiT1表达的RT-qPCR分析.
- 电子显微镜对PiT1无胚胎进行检测.
主要成果:
- 确定了PiT1/SLC20A1作为SynB.的特定细胞受体.
- PiT1调解SynB诱导的细胞融合,而PiT2则没有.
- PiT1的N端对SynB介导的核聚变至关重要.
- 在发育中的小鼠胎盘中,PiT1和SynB的表达同步升级.
- PiT1无胚胎在ST-II形成中表现出缺陷,反映了SynB无现象型.
结论:
- PiT1/SLC20A1是小鼠syncytin-B (SynB) 的功能性受体.
- 在小鼠胎盘期间,PiT1在SynB介导的syncytiotrophoblast形成中发挥着至关重要的作用.
- 这些发现为哺乳动物繁殖的内源性逆转录病毒基因的合作提供了洞察力.
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