相关实验视频
Updated: Jun 12, 2025

11:19
Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
Published on: February 20, 2017
7.4K
在Streptococcus pneumoniae中,DivIVA控制了隔膜分裂和细胞延长的动态
Jennyfer Trouve1, André Zapun1, Laure Bellard1
1Université Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France.
mBio
|September 17, 2024
概括
在Streptococcus pneumoniae中,DIVIVA蛋白调节细菌细胞分裂和延长,通过确保细胞壁均重塑. 这项研究揭示了由DIVIVA控制的丁甘氨酸合成的两个不同的阶段,这对卵形态至关重要.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 细菌细胞的形状和分裂取决于糖的合成和分裂.
- 卵座协调细胞壁组合在细胞中部区域.
- 众所周知,DivIVA是这些过程的调节者,但其机制尚不清楚.
研究的目的:
- 为了研究DIVIVA在Streptococcus pneumoniae细胞形态发生中的作用.
- 阐明DIVIVA在细菌分裂和延长中的作用机制.
主要方法:
- 集成点击基于化学的酸糖标签.
- 先进的显微镜技术:直接的随机光学重建显微镜 (dSTORM),光和刺激发射耗尽 (STED) 显微镜.
- 在模拟模型.
主要成果:
- 在细胞循环期间识别了两种不同的DIVIVA控制的丁甘氨酸重塑的阶段.
- 证明了DIVIVA在确保均隔膜裂解和外围二甲糖合成方面的作用.
- 显示了DIVIVA对延长细胞和A类青素结合蛋白对细胞延长的作用.
结论:
- 迪维瓦对于维持细胞壁重塑均和卵细胞适当的细胞分裂至关重要.
- 迪维瓦的定位表明它在感知和稳定负曲的膜区域方面发挥了作用.
- 为DIVIVA作为关键形态遗传蛋白的机制提出了假设.
相关概念视频
Role of Septins
1.7K
Septins are the recently discovered fourth major protein component of the cytoskeleton, along with microfilaments, microtubules, and intermediate filaments. These proteins can associate with other cytoskeletal filaments and carry out varied roles or can be free-floating in the cytoplasm.
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
Cellular Functions of Septins
Recent studies have revealed the multifaceted roles of septins in various cellular processes such as cytokinesis, ciliogenesis, and neurogenesis. Septins act as scaffolds and...
1.7K
Determining the Plane of Cell Division
3.3K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function.
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
3.3K
Cytoskeletal Proteins in Bacteria
3.3K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.3K
Septins
1.8K
Septins are protein filaments forming the cytoskeleton along with the microtubules, microfilaments, intermediate filaments, and other accessory proteins. In 1971 while studying the cell division cycle in mutant Saccharomyces cerevisiae Harwell et al. first identified the septin-related genes playing a crucial role in yeast cytokinesis. Fluorescence microscopy revealed that these proteins localize at the budding neck as rings. These ring-like proteins were then named Septins by John Pringle, and...
1.8K
Binary Fission
55.1K
Fission is the division of a single entity into two or more parts, which regenerate into separate entities that resemble the original. Organisms in the Archaea and Bacteria domains reproduce using binary fission, in which a parent cell splits into two parts that can each grow to the size of the original parent cell. This asexual method of reproduction produces cells that are all genetically identical.
55.1K
The Contractile Ring
6.3K
Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
6.3K

